자궁경부 상피내 종양과 침윤성 편평상피암종에서 claudin-1, claudin-4, zonula occludens-1의 발현

Abstract

=목적: 종양의 발생과 진행 과정에서 폐쇄막를 구성하는 단백질인 claudins와 ZO가 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 자궁경부의 상피내 종양과 ISCC에서 claudin-1, claudin-4, ZO-1의 발현 양상의 차이점을 알아보고자 하였다.연구 방법: 자궁경부의 생검이나 원뿔절제술을 통하여 LSIL로 진단된 22예, HSIL로 진단된 36예와 자궁적출술을 통하여 ISCC로 진단된 20예의 파라핀 포매 조직에서 면역조직화학 염색법을 이용하여 claudin-1, claudin-4, ZO-1의 발현을 분석하였다.결과: claudin-1은 LSIL의 40.9%, HSIL의 94.4%, ISCC의 20.0%에서 양성 발현이 관찰되어 HSIL에서 유의하게 높았으며 (p=0.0001), claudin-4는 LSIL의 31.8%, HSIL의 41.7%, ISCC의 25.0%에서 양성 발현이 되어 HSIL에서 가장 높았으나, 통계학적 유의성은 없었다. ZO-1은 LSIL의 13.6%, HSIL의 41.7%, ISCC의 10.0%에서 양성 발현이 관찰되어 HSIL에서 유의하게 높았다 (p=0.011).

Objective: Cell to cell and cell to extracellular matrix interaction are crucial in tumor development and progression. Tight junction proteins such as claudins and zonula occludens-1 (ZO-1) play an important role in these processes. This study was performed to investigate the difference of expressions of claudin-1, claudin-4 and ZO-1 in low grade squamous intraepithelial lesion (LSIL), high grade squamous intraepithelial lesion (HSIL), and invasive squamous cell carcinoma (ISCC) of the uterine cervix.· Methods: The expressions of claudin-1, claudin-4 and ZO-1 were evaluated using immunohistochemical staining in 78 cervical tissue specimens (LSIL 22 case, HSIL 36 case, and ISCC 20 case). Results: Claudin-1 expression was positive in 40.9% of LSIL, in 94.0% of HSIL and in 20.0% of ISCC. The expression of claudin-1 was significantly high in HSIL (p=0.0001). Claudin-4 expression was positive in 31.8% of LSIL, in 41.7% of HSIL and in 25.0% of ISCC. The expression of claudin-4 was high in HSIL, but it was not statistically different. ZO-1 expression was positive in 13.6% of LSIL, in 41.7% of HSIL, and in 25.5% of ISCC. The expression of ZO-1 was significantly high in HSIL (p=0.011). Conclusion: These results indicate increased expressions of claudin-1 and ZO-1 in the HSIL that includes cervical intraepithelial neoplasia (CIN) 2 and 3, which decrease during progression to cervical cancer.