Exosome-mediated delivery of gga-miR-20a-5p regulates immune response of chicken macrophages by targeting IFNGR2, MAPK1, MAP3K5, and MAP3K14
DC Field | Value | Language |
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dc.contributor.author | Hong, Yeojin | - |
dc.contributor.author | Heo, Jubi | - |
dc.contributor.author | Kang, Suyeon | - |
dc.contributor.author | Vu, Thi Hao | - |
dc.contributor.author | Lillehoj, Hyun S. | - |
dc.contributor.author | Hong, Yeong Ho | - |
dc.date.accessioned | 2023-09-19T07:43:57Z | - |
dc.date.available | 2023-09-19T07:43:57Z | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 2765-0189 | - |
dc.identifier.issn | 2765-0235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67682 | - |
dc.description.abstract | Objective: This study aims to evaluate the target genes of gga-miR-20a-5p and the regulated immune responses in the chicken macrophage cell line, HD11, by the exosome-mediated delivery of miR-20a-5p. Methods: Exosomes were purified from the chicken macrophage cell line HD11. Then, mimic gga-miR-20p or negative control miRNA were internalized into HD11 exosomes. HD11 cells were transfected with gga-miR-20a-5p or negative control miRNA containing exosomes. After 44 h of transfection, cells were incubated with or without 5 μg/mL poly(I:C) for 4 h. Then, expression of target genes and cytokines was evaluated by quantitative real-time polymerase chain reaction. Results: Using a luciferase reporter assay, we identified that gga-miR-20a-5p directly targeted interferon gamma receptor 2 (IFNGR2), mitogen-activated protein kinase 1 (MAPK1), mitogen-activated protein kinase kinase kinase 5 (MAP3K5), and mitogen-activated protein kinase kinase kinase 14 (MAP3K14). Moreover, the exosome-mediated delivery of gga-miR-20a-5p successfully repressed the expression of IFNGR2, MAPK1, MAP3K5, and MAP3K14 in HD11 cells. The expressions of interferon-stimulated genes (MX dynamin like GTPase 1 [MX1], eukaryotic translation initiation factor 2A [EIF2A], and oligoadenylate synthase-like [OASL]) and proinflammatory cytokines (interferon-gamma [IFNG], interleukin-1 beta [IL1B], and tumor necrosis factor-alpha [TNFA]) were also downregulated by exosomal miR-20a-5p. In addition, the proliferation of HD11 cells was increased by exosomal miR-20a-5p. Conclusion: The exosome-mediated delivery of gga-miR-20a-5p regulated immune responses by controlling the MAPK and apoptotic signaling pathways. Furthermore, we expected that exosomal miR-20a-5p could maintain immune homeostasis against highly pathogenic avian influenza virus H5N1 infection by regulating the expression of proinflammatory cytokines and cell death. Copyright © 2023 by Animal Bioscience. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Asian-Australasian Association of Animal Production Societies | - |
dc.title | Exosome-mediated delivery of gga-miR-20a-5p regulates immune response of chicken macrophages by targeting IFNGR2, MAPK1, MAP3K5, and MAP3K14 | - |
dc.title.alternative | Exosome-mediated delivery of gga-miR-20a-5p regulates immune response of chicken macrophages by targeting IFNGR2, MAPK1, MAP3K5, and MAP3K14 | - |
dc.type | Article | - |
dc.identifier.doi | 10.5713/ab.22.0373 | - |
dc.identifier.bibliographicCitation | Animal Bioscience, v.36, no.6, pp 851 - 860 | - |
dc.identifier.kciid | ART002960461 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000988204800003 | - |
dc.identifier.scopusid | 2-s2.0-85159669890 | - |
dc.citation.endPage | 860 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 851 | - |
dc.citation.title | Animal Bioscience | - |
dc.citation.volume | 36 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Exosomes | - |
dc.subject.keywordAuthor | MAPK Signaling Pathway | - |
dc.subject.keywordAuthor | miR-20a-5p | - |
dc.subject.keywordAuthor | miRNAs | - |
dc.subject.keywordPlus | TOLL-LIKE RECEPTOR | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | EXTRACELLULAR VESICLES | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | COMMUNICATION | - |
dc.subject.keywordPlus | BIOGENESIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MICRORNAS | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Dairy & Animal Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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