Fabrication of graphene oxide-based pretreatment filter and Electrochemical-CRISPR biosensor for the field-ready cyanobacteria monitoring system
DC Field | Value | Language |
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dc.contributor.author | Kim, Gahyeon | - |
dc.contributor.author | Li, Yun Guang | - |
dc.contributor.author | Seo, Yoseph | - |
dc.contributor.author | Baek, Changyoon | - |
dc.contributor.author | Choi, Jin-Ha | - |
dc.contributor.author | Park, Hyunjun | - |
dc.contributor.author | An, Jeongyun | - |
dc.contributor.author | Lee, Myoungro | - |
dc.contributor.author | Noh, Seungwoo | - |
dc.contributor.author | Min, Junhong | - |
dc.contributor.author | Lee, Taek | - |
dc.date.accessioned | 2023-09-26T10:40:53Z | - |
dc.date.available | 2023-09-26T10:40:53Z | - |
dc.date.issued | 2023-10 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67768 | - |
dc.description.abstract | Microcystis aeruginosa (M. aeruginosa) cause the eutrophication of lakes and rivers. To effectively control the overgrowth of M. aeruginosa, a suitable measurement method should be required in the aquatic fields. To address this, we developed a field-ready cyanobacterial pretreatment device and an electrochemical clustered regularly interspaced short palindromic repeats (EC-CRISPR) biosensor. The cyanobacterial pretreatment device consists of a syringe, glass bead, and graphene oxide (GO) bead. Then, the M. aeruginosa dissolved in the freshwater sample was added to fabricated filter. After filtration, the purified gene was loaded onto a CRISPR-based electrochemical biosensor chip to detect M. aeruginosa gene fragments. The biosensor was composed of CRISPR/Cpf1 protein conjugated with MXene on an Au microgap electrode (AuMGE) integrated into a printed circuit board (PCB). This AuMGE/PCB system maximizes the signal-to-noise ratio, which controls the working and counter electrode areas requiring only 3 μL samples to obtain high reliability. Using the extracted M. aeruginosa gene with a pre-treatment filter, the CRISPR biosensor showed a limit of detection of 0.089 pg/μl in fresh water. Moreover, selectivity test and matrix condition test carried out using the EC-CRISPR biosensor. These handheld pre-treatment kit and biosensors can enable field-ready detection of CyanoHABs. © 2023 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Fabrication of graphene oxide-based pretreatment filter and Electrochemical-CRISPR biosensor for the field-ready cyanobacteria monitoring system | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2023.115474 | - |
dc.identifier.bibliographicCitation | Biosensors and Bioelectronics, v.237 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 001058900000001 | - |
dc.identifier.scopusid | 2-s2.0-85163298649 | - |
dc.citation.title | Biosensors and Bioelectronics | - |
dc.citation.volume | 237 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Aquatic ecosystem monitoring | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Cyanobacteria | - |
dc.subject.keywordAuthor | Hand-held filter | - |
dc.subject.keywordAuthor | PCR-Free detection | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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