Roles of the pepper JAZ protein CaJAZ1-03 and its interacting partner RING-type E3 ligase CaASRF1 in regulating ABA signaling and drought responses
DC Field | Value | Language |
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dc.contributor.author | Koh, Haeji | - |
dc.contributor.author | Joo, Hyunhee | - |
dc.contributor.author | Lim, Chae Woo | - |
dc.contributor.author | Lee, Sung Chul | - |
dc.date.accessioned | 2023-09-28T13:40:52Z | - |
dc.date.available | 2023-09-28T13:40:52Z | - |
dc.date.issued | 2023-11 | - |
dc.identifier.issn | 0140-7791 | - |
dc.identifier.issn | 1365-3040 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/67888 | - |
dc.description.abstract | Plants have developed various defense mechanisms against environmental stresses by regulating hormone signaling. Jasmonic acid (JA) is a major phytohormone associated with plant defense responses. JASMONATE ZIM-DOMAIN (JAZ) proteins play a regulatory role in repressing JA signaling, impacting plant responses to both biotic and abiotic stresses. Here, we isolated 7 JAZ genes in pepper and selected CA03g31030, a Capsicum annuum JAZ1-03 (CaJAZ1-03) gene, for further study based on its expression level in response to abiotic stresses. Through virus-induced gene silencing (VIGS) in pepper and overexpression in transgenic Arabidopsis plants, we established the functional role of CaJAZ1-03. Functional studies revealed that CaJAZ1-03 dampens abscisic acid (ABA) signaling and drought stress responses. The cell-free degradation assay showed faster degradation of CaJAZ1-03 in drought- or ABA-treated pepper leaves compared to healthy leaves. Conversely, CaJAZ1-03 was completely preserved under MG132 treatment, indicating that CaJAZ1-03 stability is modulated via the ubiquitin-26s proteasome pathway. We also found that the pepper RING-type E3 ligase CaASRF1 interacts with and ubiquitinates CaJAZ1-03. Additional cell-free degradation assays revealed a negative correlation between CaJAZ1-03 and CaASRF1 expression levels. Collectively, these findings suggest that CaJAZ1-03 negatively regulates ABA signaling and drought responses and that its protein stability is modulated by CaASRF1. | - |
dc.format.extent | 16 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Roles of the pepper JAZ protein CaJAZ1-03 and its interacting partner RING-type E3 ligase CaASRF1 in regulating ABA signaling and drought responses | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/pce.14692 | - |
dc.identifier.bibliographicCitation | PLANT CELL AND ENVIRONMENT, v.46, no.11, pp 3242 - 3257 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 001045466500001 | - |
dc.identifier.scopusid | 2-s2.0-85167665691 | - |
dc.citation.endPage | 3257 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 3242 | - |
dc.citation.title | PLANT CELL AND ENVIRONMENT | - |
dc.citation.volume | 46 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | abscisic acid | - |
dc.subject.keywordAuthor | drought | - |
dc.subject.keywordAuthor | jasmonic acid | - |
dc.subject.keywordAuthor | protein stability | - |
dc.subject.keywordAuthor | ubiquitination | - |
dc.subject.keywordPlus | ABSCISIC-ACID | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | NEGATIVE REGULATOR | - |
dc.subject.keywordPlus | STRESS-RESPONSE | - |
dc.subject.keywordPlus | JASMONATE | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | TOLERANCE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | ACTS | - |
dc.subject.keywordPlus | DEFENSE | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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