Silymarin-Functionalized Selenium Nanoparticles Prevent LPS- Induced Inflammatory Response in RAW264.7 Cells through Downregulation of the PI3K/Akt/NF-Kappa B Pathway
DC Field | Value | Language |
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dc.contributor.author | Mi, Xiao-jie | - |
dc.contributor.author | Le, Ha-Minh | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Park, Hye-Ryung | - |
dc.contributor.author | Kim, Yeon-Ju | - |
dc.date.accessioned | 2023-10-26T05:29:43Z | - |
dc.date.available | 2023-10-26T05:29:43Z | - |
dc.date.issued | 2022-11 | - |
dc.identifier.issn | 2470-1343 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68290 | - |
dc.description.abstract | Silymarin exhibits an anti-inflammatory property in various cancers and inflammatory diseases. In our previous work, silymarin-mediated selenium nanoparticles (SeNPs) (Si-SeNPs) were developed using a green synthesis technique, and its potential as an anticancer agent was confirmed. In order to further examine the extended comprehensive potential of Si-SeNPs, this investigation focuses on studying the enhanced anti-inflammatory effect of Si-SeNPs in lipopolysaccharide (LPS)-stimulated RAW264.7 macro-phages. Enzyme-linked immunosorbent assay and quantitative reverse transcription-polymerase chain reaction were used to evaluate the expression of pro-inflammatory mediators and cytokines. Western blotting and immunofluorescence assays were conducted to assess the protein expression of p-PI3K, p-Akt, p-NF-kappa B, and p-I kappa B alpha. Compared to silymarin, Si-SeNPs exhibited a significantly increased inhibitory effect on LPS-induced release of nitric oxide and the expression of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) in RAW264.7 cells. A western blot assay indicated that Si-SeNPs downregulated the PI3K/Akt and NF-kappa B signaling pathways. The immunofluorescence assay suggested that Si-SeNPs inhibited the nuclear translocation and the activation of NF-kappa B. In addition, 740 Y-P (PI3K agonist) was used to demonstrate that activating the PI3K/Akt signal could partially reverse the inflammatory response, suggesting a causal role of the PI3K/Akt signaling pathway in the anti-inflammatory effect of Si-SeNPs. Consequently, these findings indicate that Si-SeNPs could be a functional agent of the attenuation of LPS-induced inflammatory responses in RAW264.7 macrophages through inhibiting the PI3K/Akt/NF-kappa B signaling pathway. In addition, biosynthesized Si-SeNPs could be more effective at reducing inflammation than only silymarin extracts. Thus, this study lays an experimental foundation for the clinical application of using biosynthesized SeNPs as a novel candidate in the field of inflammation-associated diseases. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Silymarin-Functionalized Selenium Nanoparticles Prevent LPS- Induced Inflammatory Response in RAW264.7 Cells through Downregulation of the PI3K/Akt/NF-Kappa B Pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acsomega.2c04140 | - |
dc.identifier.bibliographicCitation | ACS OMEGA, v.7, no.47, pp 42723 - 42732 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000890226200001 | - |
dc.identifier.scopusid | 2-s2.0-85142643231 | - |
dc.citation.endPage | 42732 | - |
dc.citation.number | 47 | - |
dc.citation.startPage | 42723 | - |
dc.citation.title | ACS OMEGA | - |
dc.citation.volume | 7 | - |
dc.type.docType | Article; Early Access | - |
dc.publisher.location | 미국 | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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