First Detection of Enterovirus D68 in Korean Children, September 2022
DC Field | Value | Language |
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dc.contributor.author | Yun, Ki Wook | - |
dc.contributor.author | Ahn, Bin | - |
dc.contributor.author | Choi, Sung Hwan | - |
dc.contributor.author | Kang, Da Yeon | - |
dc.contributor.author | Kim, Taek Soo | - |
dc.contributor.author | Lee, Mi Kyung | - |
dc.contributor.author | Park, Kyoung Un | - |
dc.contributor.author | Choi, Eun Hwa | - |
dc.date.accessioned | 2023-11-07T03:42:05Z | - |
dc.date.available | 2023-11-07T03:42:05Z | - |
dc.date.issued | 2023-12 | - |
dc.identifier.issn | 2093-2340 | - |
dc.identifier.issn | 2092-6448 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68481 | - |
dc.description.abstract | Background: Enterovirus D68 (EV-D68) is a re-emerging pathogen that is particularly common in children and may cause asthma-like respiratory infection and acute flaccid myelitis. However, in Korea, EV-D68 has never been reported thus far. This study aimed to identify EV-D68 from nasopharyngeal aspirates (NPAs) in Korean children with a respiratory tract infection. Materials and Methods: The EV-D68 reference strain was purchased and blindly used to assess the detection ability of three commercial and one in-house mRT-PCR kit in 2018. Then, we selected children whose specimens were positive for human rhinovirus (HRV) and/or enterovirus (EV) by Allplex mRT-PCR (Seegene, Inc., Seoul, Korea) from April to December 2022. Total RNA was extracted from NPAs, and a partial 5'-UTR gene was amplified and sequenced for the identification of HRV/EV species. Additionally, PCR targeting the VP1 gene was performed to assess EV-D68-positive NPAs, followed by sequencing. Phylogenetic analysis and comparison of amino acid sequence alignments were performed using a partial VP1 gene of our and recent international EV-D68 strains. Results: Among the mRT-PCR kits tested, only the in-house kit was able to detect EV-D68 in 2018. However, we detected three EV-D68 strains among children hospitalized with fever and/or respiratory symptoms in September - December 2022 who tested positive for EV by the Allplex kit. Two of them were healthy toddlers with lower respiratory infections accompanied by new-onset wheezing but no neurologic complications. Among 34 children with lower respiratory infection who tested positive for HRV during the same period, EV-D68 was not detected. Phylogenetic analysis revealed that the first Korean EV-D68 belonged to subclade B3. Amino acid sequence alignment of international subclade B3 EV-D68 strains also showed that our strain is genetically more related to those from Europe than those from Japan. Conclusion: We first detected EV-D68 in three Korean children who had EV detected by the Allplex mRT-PCR kit in 2022. EV-D68 also circulated in Korea in fall 2022, but the prevalence and severity seemed to be lower than those in previous reports from other countries. © 2023 Korean Society for Antimicrobial Therapy. All rights reserved. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society of Infectious Diseases, Korean Society for Antimicrobial Therapy, Korean Society for AIDS | - |
dc.title | First Detection of Enterovirus D68 in Korean Children, September 2022 | - |
dc.title.alternative | First Detection of Enterovirus D68 in Korean Children, September 2022 | - |
dc.type | Article | - |
dc.identifier.doi | 10.3947/ic.2023.0036 | - |
dc.identifier.bibliographicCitation | Infection and Chemotherapy, v.55, no.4, pp 422 - 430 | - |
dc.identifier.kciid | ART003035441 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 001136731200015 | - |
dc.identifier.scopusid | 2-s2.0-85171451222 | - |
dc.citation.endPage | 430 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 422 | - |
dc.citation.title | Infection and Chemotherapy | - |
dc.citation.volume | 55 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Children | - |
dc.subject.keywordAuthor | Enterovirus D68 | - |
dc.subject.keywordAuthor | Respiratory infection | - |
dc.subject.keywordPlus | INCREASE | - |
dc.subject.keywordPlus | ILLNESS | - |
dc.subject.keywordPlus | SPAIN | - |
dc.relation.journalResearchArea | Infectious Diseases | - |
dc.relation.journalWebOfScienceCategory | Infectious Diseases | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | esci | - |
dc.description.journalRegisteredClass | kci | - |
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