Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sun-Ki | - |
dc.contributor.author | Groom, Joseph | - |
dc.contributor.author | Chung, Daehwan | - |
dc.contributor.author | Elkins, James | - |
dc.contributor.author | Westpheling, Janet | - |
dc.date.accessioned | 2023-11-07T07:41:20Z | - |
dc.date.available | 2023-11-07T07:41:20Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68503 | - |
dc.description.abstract | Background: Resistance to deconstruction is a major limitation to the use of lignocellulosic biomass as a substrate for the production of fuels and chemicals. Consolidated bioprocessing (CBP), the use of microbes for the simultaneous hydrolysis of lignocellulose into soluble sugars and fermentation of the resulting sugars to products of interest, is a potential solution to this obstacle. The pretreatment of plant biomass, however, releases compounds that are inhibitory to the growth of microbes used for CBP. Results: Heterologous expression of the Thermoanaerobacter pseudethanolicus 39E bdhA gene, that encodes an alcohol dehydrogenase, in Clostridium thermocellum significantly increased resistance to furan derivatives at concentrations found in acid-pretreated biomass. The mechanism of detoxification of hydroxymethylfurfural was shown to be primarily reduction using NADPH as the cofactor. In addition, we report the construction of new expression vectors for homologous and heterologous expression in C. thermocellum. These vectors use regulatory signals from both C. bescii (the S-layer promoter) and C. thermocellum (the enolase promoter) shown to efficiently drive expression of the BdhA enzyme. Conclusions: Toxic compounds present in lignocellulose hydrolysates that inhibit cell growth and product formation are obstacles to the commercialization of fuels and chemicals from biomass. Expression of genes that reduce the effect of these inhibitors, such as furan derivatives, will serve to enable commercial processes using plant biomass for the production of fuels and chemicals. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s13068-017-0750-z | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY FOR BIOFUELS, v.10 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000397441700001 | - |
dc.identifier.scopusid | 2-s2.0-85016067625 | - |
dc.citation.title | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.volume | 10 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Consolidated bioprocessing | - |
dc.subject.keywordAuthor | Clostridium thermocellum | - |
dc.subject.keywordAuthor | Butanol dehydrogenase | - |
dc.subject.keywordAuthor | Furfural | - |
dc.subject.keywordAuthor | 5-hydroxymethyl-2-furfural | - |
dc.subject.keywordPlus | FURAN ALDEHYDE DETOXIFICATION | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | CALDICELLULOSIRUPTOR-BESCII | - |
dc.subject.keywordPlus | PLANT BIOMASS | - |
dc.subject.keywordPlus | ANAEROCELLUM-THERMOPHILUM | - |
dc.subject.keywordPlus | ETHANOL YIELDS | - |
dc.subject.keywordPlus | DSM 6725 | - |
dc.subject.keywordPlus | TOLERANCE | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | HMF | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.