The Protective Role of Exosome-Derived MicroRNAs and Proteins from Human Breast Milk against Infectious Agents
DC Field | Value | Language |
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dc.contributor.author | Kim, Ki-Uk | - |
dc.contributor.author | Han, Kyusun | - |
dc.contributor.author | Kim, Jisu | - |
dc.contributor.author | Kwon, Da Hyeon | - |
dc.contributor.author | Ji, Yong Woo | - |
dc.contributor.author | Yi, Dae Yong | - |
dc.contributor.author | Min, Hyeyoung | - |
dc.date.accessioned | 2023-12-01T04:40:40Z | - |
dc.date.available | 2023-12-01T04:40:40Z | - |
dc.date.issued | 2023-05 | - |
dc.identifier.issn | 2218-1989 | - |
dc.identifier.issn | 2218-1989 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68723 | - |
dc.description.abstract | Human breast milk (HBM)-derived exosomes contain various biological and immunological components. However, comprehensive immune-related and antimicrobial factor analysis requires transcriptomic, proteomic, and multiple databases for functional analyses, and has yet to be conducted. Therefore, we isolated and confirmed HBM-derived exosomes by detecting specific markers and examining their morphology using western blot and transmission electron microscopy. Moreover, we implemented small RNA sequencing and liquid chromatography-mass spectrometry to investigate substances within the HBM-derived exosomes and their roles in combating pathogenic effects, identifying 208 miRNAs and 377 proteins associated with immunological pathways and diseases. Integrated omics analyses identified a connection between the exosomal substances and microbial infections. In addition, gene ontology and the Kyoto Encyclopedia of Genes and Genomes pathway analyses demonstrated that HBM-derived exosomal miRNA and proteins influence immune-related functions and pathogenic infections. Finally, protein–protein interaction analysis identified three primary proteins (ICAM1, TLR2, and FN1) associated with microbial infections mediating pro-inflammation, controlling infection, and facilitating microbial elimination. Our findings determine that HBM-derived exosomes modulate the immune system and could offer therapeutic strategies for regulating pathogenic microbial infection. © 2023 by the authors. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | The Protective Role of Exosome-Derived MicroRNAs and Proteins from Human Breast Milk against Infectious Agents | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/metabo13050635 | - |
dc.identifier.bibliographicCitation | Metabolites, v.13, no.5 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000997356800001 | - |
dc.identifier.scopusid | 2-s2.0-85160407442 | - |
dc.citation.number | 5 | - |
dc.citation.title | Metabolites | - |
dc.citation.volume | 13 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | exosome | - |
dc.subject.keywordAuthor | human breast milk | - |
dc.subject.keywordAuthor | microbial defense | - |
dc.subject.keywordAuthor | proteomics | - |
dc.subject.keywordAuthor | small RNA sequencing | - |
dc.subject.keywordPlus | MEDIATED TRANSFER | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | TOLERANCE | - |
dc.subject.keywordPlus | MIR-146A | - |
dc.subject.keywordPlus | EBNA1 | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | STAT1 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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