Sphingobium tyrosinilyticum sp nov., a tyrosine hydrolyzing bacterium isolated from Korean radish garden

Abstract

A yellow-pigmented novel bacterial strain, MAH-12(T), was isolated from a soil sample of Korean radish garden and was characterized using a polyphasic approach. Cells were Gram-staining negative, non-motile and rod-shaped. The strain was aerobic, catalase positive, optimum growth temperature and pH were 28-30 degrees C and 6.0, respectively. The novel strain is able to hydrolyze L-tyrosine, starch, esculin and 4-nitrophenyl-beta-D-galactopyranoside. On the basis of 16S rRNA gene sequence analysis, strain MAH-12(T) belongs to the genus Sphingobium and is most closely related to several Sphingobium type strains (97.2-97.8%). In DNA-DNA hybridization tests, the DNA relatedness between strain MAH-12(T) and its closest phylogenetic neighbors was below 45.0%. The DNA G+C content was 64.0 mol% and the predominant respiratory quinone was ubiquinone-10. The major cellular fatty acids were summed feature 8 (C-18:1 omega 7c and/or C-18(:1) omega 6c) and C-16(:0). The DNA-DNA hybridization results and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain MAH-12(T) represented a novel species within the genus Sphingobium, for which the name Sphingobium tyrosinilyticum is proposed. The type strain is MAH-12(T) (=KACC 19297(T)=CGMCC 1.16225(T)). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain MAH-12(T) is KY964278 and the digital protologue database taxon number of strain MAH-12(T) is TA00463.