Nanoplasmonic assay platforms for reproducible SERS detection of Alzheimer's disease biomarker
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dang, H. | - |
dc.contributor.author | Joung, Y. | - |
dc.contributor.author | Jeong, C. | - |
dc.contributor.author | Jeon, C.S. | - |
dc.contributor.author | Pyun, S.H. | - |
dc.contributor.author | Park, S.-G. | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.accessioned | 2024-01-08T21:39:01Z | - |
dc.date.available | 2024-01-08T21:39:01Z | - |
dc.date.issued | 2023-05 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.issn | 1229-5949 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69571 | - |
dc.description.abstract | With the recent developments in high-sensitivity optical detection technologies, many studies have been conducted to accurately detect biomarkers with low concentrations of 1.0 pM or less as well as apply them to in vitro diagnostics. The tubulin-associated unit (tau-381) protein, a biomarker of Alzheimer's disease, is one such representative example, and its cut-off value reported in clinical practice is 5.5 fM. Therefore, a robust sensing technology that can stably detect such low concentrations of biomarkers is needed. In this study, tau-381 was detected with high sensitivity and reproducibility by a plasmonic Au nanopopcorn substrate fabricated via thermal evaporation. Here, aptamer DNAs labeled with Raman reporters on the terminal were used as the receptors. The plasmonic nanopopcorn substrate used in this study is composed of uniform gold nanoparticles (AuNPs) of average size 64 nm. The reproducibility was significantly improved through more uniform nanogaps than those formed by aggregation of AuNPs in solution. An assay was conducted by first reacting tau-381 with the corresponding aptamers, and the remaining aptamer DNAs were then reacted with capture DNAs immobilized on the surface of the Au substrate. The assay results for tau-381 showed a limit of detection value of 2.2 fM, which is below the cut-off value (5.5 fM). © 2023 Korean Chemical Society, Seoul & Wiley-VCH GmbH. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | John Wiley and Sons Inc | - |
dc.title | Nanoplasmonic assay platforms for reproducible SERS detection of Alzheimer's disease biomarker | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/bkcs.12679 | - |
dc.identifier.bibliographicCitation | Bulletin of the Korean Chemical Society, v.44, no.5, pp 441 - 448 | - |
dc.identifier.kciid | ART002959232 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000923329600001 | - |
dc.identifier.scopusid | 2-s2.0-85147277210 | - |
dc.citation.endPage | 448 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 441 | - |
dc.citation.title | Bulletin of the Korean Chemical Society | - |
dc.citation.volume | 44 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | high sensitivity | - |
dc.subject.keywordAuthor | immunoassay | - |
dc.subject.keywordAuthor | nanopopcorn substrate | - |
dc.subject.keywordAuthor | reproducibility | - |
dc.subject.keywordAuthor | surface-enhanced Raman scattering | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.