A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Hee-Yeon | - |
dc.contributor.author | Lee, Younghay | - |
dc.contributor.author | Yoon, Hee-Soo | - |
dc.contributor.author | Kim, Yu-Hee | - |
dc.contributor.author | Cho, Kyong-A | - |
dc.contributor.author | Woo, So-Youn | - |
dc.contributor.author | Kim, Han Sun | - |
dc.contributor.author | Park, Bo-Young | - |
dc.contributor.author | Jung, Sung-Chul | - |
dc.contributor.author | Jo, Inho | - |
dc.contributor.author | Park, Woo-Jae | - |
dc.contributor.author | Park, Joo-Won | - |
dc.contributor.author | Ryu, Kyung-Ha | - |
dc.date.accessioned | 2024-01-08T22:36:49Z | - |
dc.date.available | 2024-01-08T22:36:49Z | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.issn | 2212-5469 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69617 | - |
dc.description.abstract | BACKGROUND: The advantages of tonsil-derived mesenchymal stem cells (TMSCs) over other mesenchymal stem cells (MSCs) include higher proliferation rates, various differentiation potentials, efficient immune-modulating capacity, and ease of obtainment. Specifically, TMSCs have been shown to differentiate into the endodermal lineage. Estrogen deficiency is a major cause of postmenopausal osteoporosis and is associated with higher incidences of ischemic heart disease and cerebrovascular attacks during the postmenopausal period. Therefore, stem cell-derived, estrogen-secreting cells might be used for estrogen deficiency. METHODS: Here, we developed a novel method that utilizes retinoic acid, insulin-like growth factor-1, basic fibroblast growth factor, and dexamethasone to evaluate the differentiating potential of TMSCs into estrogen-secreting cells. The efficacy of the novel differentiating method for generation of estrogen-secreting cells was also evaluated with bone marrow- and adipose tissue-derived MSCs. RESULTS: Incubating TMSCs in differentiating media induced the gene expression of cytochrome P450 19A1 (CYP19A1), which plays a key role in estrogen biosynthesis, and increased 17 beta-estradiol secretion upon testosterone addition. Furthermore, CYP11A1, CYP17A1, and 3 beta-hydroxysteroid dehydrogenase type-1 gene expression levels were significantly increased in TMSCs. In bone marrow-derived and adipose tissue-derived MSCs, this differentiation method also induced the gene expression of CYP19A1, but not CYP17A1, suggesting TMSCs are a superior source for estrogen secretion. CONCLUSION: These results imply that TMSCs can differentiate into functional estrogen-secreting cells, thus providing a novel, alternative cell therapy for estrogen deficiency. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | - |
dc.title | A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s13770-020-00307-y | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.18, no.2, pp 253 - 264 | - |
dc.identifier.kciid | ART002702744 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000584859300001 | - |
dc.identifier.scopusid | 2-s2.0-85094144327 | - |
dc.citation.endPage | 264 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 253 | - |
dc.citation.title | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.volume | 18 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Estrogen | - |
dc.subject.keywordAuthor | Tonsil | - |
dc.subject.keywordAuthor | Mesenchymal stem cell | - |
dc.subject.keywordAuthor | Secretion | - |
dc.subject.keywordAuthor | Cytochrome P450 family 19 subfamily A member 1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BETA | - |
dc.subject.keywordPlus | DEFICIENCY | - |
dc.subject.keywordPlus | AROMATASE | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | CANCER | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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