Biochemical Characterization of Echinococcus multilocularis Antigen B3 Reveals Insight into Adaptation and Maintenance of Parasitic Homeostasis at the Host-Parasite Interface
DC Field | Value | Language |
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dc.contributor.author | Ahn, Chun-Seob | - |
dc.contributor.author | Kim, Jeong-Geun | - |
dc.contributor.author | Han, Xiumin | - |
dc.contributor.author | Bae, Young-An | - |
dc.contributor.author | Park, Woo-Jae | - |
dc.contributor.author | Kang, Insug | - |
dc.contributor.author | Wang, Hu | - |
dc.contributor.author | Kong, Yoon | - |
dc.date.accessioned | 2024-01-08T22:37:10Z | - |
dc.date.available | 2024-01-08T22:37:10Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.issn | 1535-3907 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69630 | - |
dc.description.abstract | Alveolar echinococcosis (AE) caused by Echinococcus multilocularis metacestode is frequently associated with deleterious zoonotic helminthiasis. The growth patterns and morphological features of AE, such as invasion of the liver parenchyme and multiplication into multivesiculated masses, are similar to those of malignant tumors. AE has been increasingly detected in several regions of Europe, North America, Central Asia, and northwestern China. An isoform of E. multilocularis antigen B3 (EmAgB3) shows a specific immunoreactivity against patient sera of active-stage AE, Suggesting that EmAgB3 might play important roles during adaptation of the parasite to hosts. However, expression patterns and biochemical properties of EmAgB3 remained elusive. The protein profile and nature of component proteins of E. multilocularis hydatid fluid (EmHF) have never been addressed. In this study, we conducted proteome analysis of EmHF of AE cysts harvested from immunocompetent mice. We observed the molecular and biochemical properties of EmAgB3, including differential transcription patterns of paralogous genes, macromolecular protein status by self-assembly, distinct oligomeric states according to individual anatomical compartments of the worm, and hydrophobic ligand-binding protein activity. We also demonstrated tissue expression patterns of EmAgB3 transcript and protein. EmAgB3 might participate in immune response and recruitment of essential host lipids at the host-parasite interface. Our results might contribute to an in depth understanding of the biophysical and biological features of EmAgB3, thus providing insights into the design of novel targets to control AE. | - |
dc.format.extent | 18 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Biochemical Characterization of Echinococcus multilocularis Antigen B3 Reveals Insight into Adaptation and Maintenance of Parasitic Homeostasis at the Host-Parasite Interface | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.jproteome.6b00799 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PROTEOME RESEARCH, v.16, no.2, pp 806 - 823 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000393539600042 | - |
dc.identifier.scopusid | 2-s2.0-85011702097 | - |
dc.citation.endPage | 823 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 806 | - |
dc.citation.title | JOURNAL OF PROTEOME RESEARCH | - |
dc.citation.volume | 16 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Echinococcus multilocularis | - |
dc.subject.keywordAuthor | alveolar echinococcosis | - |
dc.subject.keywordAuthor | hydatid fluid | - |
dc.subject.keywordAuthor | proteome | - |
dc.subject.keywordAuthor | antigen B3 | - |
dc.subject.keywordAuthor | expressional regulation | - |
dc.subject.keywordAuthor | multimeric states | - |
dc.subject.keywordAuthor | hydrophobic ligand-binding protein | - |
dc.subject.keywordPlus | TAENIA-SOLIUM METACESTODE | - |
dc.subject.keywordPlus | LIGAND-BINDING-PROTEIN | - |
dc.subject.keywordPlus | ALVEOLAR ECHINOCOCCOSIS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EXCRETORY/SECRETORY-PRODUCTS | - |
dc.subject.keywordPlus | CYSTIC ECHINOCOCCOSIS | - |
dc.subject.keywordPlus | ANTIBODY-RESPONSES | - |
dc.subject.keywordPlus | INTERMEDIATE HOST | - |
dc.subject.keywordPlus | DUPLICATED GENES | - |
dc.subject.keywordPlus | HYDATID FLUID | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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