Functional Equivalency in Human Somatic Cell Nuclear Transfer-Derived Endothelial Cells
DC Field | Value | Language |
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dc.contributor.author | Park, Soon-Jung | - |
dc.contributor.author | Lee, Ji-Heon | - |
dc.contributor.author | Lee, Seul-Gi | - |
dc.contributor.author | Lee, Jeoung Eun | - |
dc.contributor.author | Seo, Joseph | - |
dc.contributor.author | Choi, Jong Jin | - |
dc.contributor.author | Jung, Taek-Hee | - |
dc.contributor.author | Chung, Eun-Bin | - |
dc.contributor.author | Kim, Ha Na | - |
dc.contributor.author | Ju, Jongil | - |
dc.contributor.author | Song, Yun-Ho | - |
dc.contributor.author | Chung, Hyung-Min | - |
dc.contributor.author | Lee, Dong Ryul | - |
dc.contributor.author | Moon, Sung-Hwan | - |
dc.date.accessioned | 2024-01-09T03:31:15Z | - |
dc.date.available | 2024-01-09T03:31:15Z | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.issn | 1549-4918 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69807 | - |
dc.description.abstract | The derivation of human embryonic stem cells (hESCs) by somatic cell nuclear transfer (SCNT) has prompted a re-emerging interest in using such cells for therapeutic cloning. Despite recent advancements in derivation protocols, the functional potential of CHA-NT4 derived cells is yet to be elucidated. For this reason, this study sought to differentiate CHA-NT4 cells toward an endothelial lineage in order to evaluate in vitro and in vivo functionality. To initial differentiation, embryoid body formation of CHA-NT4 was mediated by concave microwell system which was optimized for hESC-endothelial cell (EC) differentiation. The isolated CD31+ cells exhibited hallmark endothelial characteristics in terms of morphology, tubule formation, and ac-LDL uptake. Furthermore, CHA-NT4-derived EC (human nuclear transfer [hNT]-ESC-EC) transplantation in hind limb ischemic mice rescued the hind limb and restored blood perfusion. These findings suggest that hNT-ESC-EC are functionally equivalent to hESC-ECs, warranting further study of CHA-NT4 derivatives in comparison to other well established pluripotent stem cell lines. This revival of human SCNT-ESC research may lead to interesting insights into cellular behavior in relation to donor profile, mitochondrial DNA, and oocyte quality. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Functional Equivalency in Human Somatic Cell Nuclear Transfer-Derived Endothelial Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/stem.2986 | - |
dc.identifier.bibliographicCitation | STEM CELLS, v.37, no.5, pp 623 - 630 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000466952000010 | - |
dc.identifier.scopusid | 2-s2.0-85061990483 | - |
dc.citation.endPage | 630 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 623 | - |
dc.citation.title | STEM CELLS | - |
dc.citation.volume | 37 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Human somatic cell nuclear transfer | - |
dc.subject.keywordAuthor | Endothelial cell | - |
dc.subject.keywordAuthor | Ischemia | - |
dc.subject.keywordAuthor | Cell transplantation | - |
dc.subject.keywordAuthor | Cell therapy | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | BLOOD | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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