Modification of a Purification and Expansion Method for Human Embryonic Stem Cell-Derived Cardiomyocytes
DC Field | Value | Language |
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dc.contributor.author | Park, Soon-Jung | - |
dc.contributor.author | Bae, Daekyeong | - |
dc.contributor.author | Moon, Sung-Hwan | - |
dc.contributor.author | Chung, Hyung-Min | - |
dc.date.accessioned | 2024-01-09T03:32:14Z | - |
dc.date.available | 2024-01-09T03:32:14Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.issn | 0008-6312 | - |
dc.identifier.issn | 1421-9751 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69858 | - |
dc.description.abstract | Objective:This study aimed to develop a simple and efficient purification method for human embryonic stem cell (hESC)-derived cardiomyocytes (CMs) using a low-glucose culture system. In addition, we investigated whether intercellular adhesion between single hESC-CMs plays a critical role in enhancing proliferation of purified hESC-CMs. Method: hESCs were cultured in suspension to form human embryoid bodies (hEBs) from which similar to 15% contracting clusters were derived after 15-20 days in culture. To purify CMs from contracting hEBs, we first manually isolated contracting clumps that were re-cultured on gelatin-coated plates with media containing a low concentration of glucose. The purified hESC-CMs were cultured at different densities to examine whether cell-cell contact enhances proliferation of hESC-CMs. Results: Purified CMs demonstrated spontaneous contraction and strongly expressed the CM-specific markers cardiac troponin T and slow myosin heavy chain. We investigated the purification efficiency by examining the expression levels of cardiac-related genes and the expression of MitoTracker Red dye. In addition, purified hESC-CMs in low-glucose culture demonstrated a 1.5-fold increase in their proliferative capacity compared to those cultured as single hESC-CMs. Conclusion: A low level of glucose is efficient in purifying hESC-CMs and intercellular adhesion between individual hESC-CMs plays a critical role in enhancing hESC-CM proliferation. Copyright (C) 2013 S. Karger AG, Basel | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KARGER | - |
dc.title | Modification of a Purification and Expansion Method for Human Embryonic Stem Cell-Derived Cardiomyocytes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1159/000346390 | - |
dc.identifier.bibliographicCitation | CARDIOLOGY, v.124, no.3, pp 139 - 150 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000316838800001 | - |
dc.identifier.scopusid | 2-s2.0-84874009027 | - |
dc.citation.endPage | 150 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 139 | - |
dc.citation.title | CARDIOLOGY | - |
dc.citation.volume | 124 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Cardiomyocytes | - |
dc.subject.keywordAuthor | Expansion | - |
dc.subject.keywordAuthor | Human embryonic stem cells | - |
dc.subject.keywordAuthor | Purification | - |
dc.subject.keywordPlus | GLUCOSE INDUCES APOPTOSIS | - |
dc.subject.keywordPlus | C-MYC | - |
dc.subject.keywordPlus | CARDIAC REPAIR | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.relation.journalResearchArea | Cardiovascular System & Cardiology | - |
dc.relation.journalWebOfScienceCategory | Cardiac & Cardiovascular Systems | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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