Architectural alterations of the fission yeast genome during the cell cycle
- Authors
- Tanizawa, Hideki; Kim, Kyoung-Dong; Iwasaki, Osamu; Noma, Ken-ichi
- Issue Date
- Nov-2017
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE STRUCTURAL & MOLECULAR BIOLOGY, v.24, no.11, pp 965 - 976
- Pages
- 12
- Journal Title
- NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Volume
- 24
- Number
- 11
- Start Page
- 965
- End Page
- 976
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69962
- DOI
- 10.1038/nsmb.3482
- ISSN
- 1545-9993
1545-9985
- Abstract
- Eukaryotic genomes are highly ordered through various mechanisms, including topologically associating domain (TAD) organization. We employed an in situ Hi-C approach to follow the 3D organization of the fission yeast genome during the cell cycle. We demonstrate that during mitosis, large domains of 300 kb-1 Mb are formed by condensin. This mitotic domain organization does not suddenly dissolve, but gradually diminishes until the next mitosis. By contrast, small domains of 30-40 kb that are formed by cohesin are relatively stable across the cell cycle. Condensin and cohesin mediate long-and short-range contacts, respectively, by bridging their binding sites, thereby forming the large and small domains. These domains are inversely regulated during the cell cycle but assemble independently. Our study describes the chromosomal oscillation between the formation and decay phases of the large and small domains, and we predict that the condensin-mediated domains serve as chromosomal compaction units.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69962)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.