Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyoung-Dong | - |
dc.contributor.author | Kim, Hyo-Jin | - |
dc.contributor.author | Lee, Kyung-Chang | - |
dc.contributor.author | Roe, Jung-Hye | - |
dc.date.accessioned | 2024-01-09T06:05:24Z | - |
dc.date.available | 2024-01-09T06:05:24Z | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69969 | - |
dc.description.abstract | The fission yeast Schizosaccharomyces pombe contains two CGFS-type monothiol glutaredoxins. Grx4 and Grx5, which are localized primarily in the nucleus and mitochondria, respectively. We observed involvement of Grx4 in regulating iron-responsive gene expression, which is modulated by a repressor Fep1. Lack of Grx4 caused defects not only in growth but also in the expression of both iron-uptake and iron-utilizing genes regardless of iron availability. In order to unravel how Grx4 is involved in Fep1-mediated regulation, interaction between them was investigated. Co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) revealed that Grx4 physically interacts with Fep1 in vivo. BiFC revealed localized nuclear dots produced by interaction of Grx4 with Fep1. Mutation of cysteine-172 in the CGFS motif to serine (C172S) produced effects similarly observed under Grx4 depletion, such as the loss of iron-dependent gene regulation and the absence of nuclear dots in BiFC analysis. These results suggest that the ability of Grx4 to bind iron, most likely Fe-S cofactor, could be critical in interacting with and modulating the activity of Fep1. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2011.04.069 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.408, no.4, pp 609 - 614 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000291293200018 | - |
dc.identifier.scopusid | 2-s2.0-79956202651 | - |
dc.citation.endPage | 614 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 609 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 408 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Monothiol glutaredoxin | - |
dc.subject.keywordAuthor | Fep1 | - |
dc.subject.keywordAuthor | Iron homeostasis | - |
dc.subject.keywordAuthor | Fission yeast | - |
dc.subject.keywordAuthor | CGFS motif | - |
dc.subject.keywordPlus | BIMOLECULAR FLUORESCENCE COMPLEMENTATION | - |
dc.subject.keywordPlus | BINDING FACTOR PHP4 | - |
dc.subject.keywordPlus | SCHIZOSACCHAROMYCES-POMBE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | MONOTHIOL GLUTAREDOXINS | - |
dc.subject.keywordPlus | MITOCHONDRIAL THIOREDOXIN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | SULFUR CLUSTER | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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