Primary bone-derived cells induce osteogenic differentiation without exogenous factors in human embryonic stem cells
DC Field | Value | Language |
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dc.contributor.author | Ahn, Seong Eun | - |
dc.contributor.author | Kim, Sinae | - |
dc.contributor.author | Park, Kyu Hyung | - |
dc.contributor.author | Moon, Sung Hwan | - |
dc.contributor.author | Lee, Hae Jin | - |
dc.contributor.author | Kim, Gi Jin | - |
dc.contributor.author | Lee, Young Jae | - |
dc.contributor.author | Park, Keun Hong | - |
dc.contributor.author | Cha, Kwang Yul | - |
dc.contributor.author | Chung, Hyung Min | - |
dc.date.accessioned | 2024-01-09T06:07:10Z | - |
dc.date.available | 2024-01-09T06:07:10Z | - |
dc.date.issued | 2006-02 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/69986 | - |
dc.description.abstract | We developed a new and efficient method for osteoblastic differentiation of human embryonic stem cells (hESCs) using primary bone-derived cells (PBDs). Three days after embryoid body (hEB) formation, cells were allowed to adhere to culture surface where PBDs were pre-plated and mitomycin C-treated in DMEM/F12 medium supplemented with 5% knockout serum replacement, As early as 14 days, mineralization and formation of nodule-like structures in cocultured hEBs were prominent by von Kossa and Alizarin S staining, and expressions of osteoblast-specific markers including bone sialoprotein, alkaline phosphates, osteocalcin, collagen 1, and core binding factor alpha 1 by RT-PCR. In addition, FACS analysis revealed that over 19% of the differentiated cells expressed osteocalcin. These results suggest that PBDs not only have osteogenic effects releasing osteogenic factors as bone morphogenic protein (BMP) 2 and BMP 4 but also have exerted other effects, whether chemical or physical, for the differentiation of hESCs. (c) 2005 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Primary bone-derived cells induce osteogenic differentiation without exogenous factors in human embryonic stem cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2005.12.020 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.340, no.2, pp 403 - 408 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000234731800008 | - |
dc.identifier.scopusid | 2-s2.0-29644436239 | - |
dc.citation.endPage | 408 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 403 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 340 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | human embryonic stem cells | - |
dc.subject.keywordAuthor | primary bone-derived cells | - |
dc.subject.keywordAuthor | coculture | - |
dc.subject.keywordAuthor | osteogenic differentiation | - |
dc.subject.keywordPlus | IN-VITRO DIFFERENTIATION | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | REGULATORS | - |
dc.subject.keywordPlus | GENE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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