Identification of galacturonic acid-rich polysaccharide with intestinal immune system modulating activity via Peyer's patch from postbiotics of Phellinus linteus mycelial submerged culture
DC Field | Value | Language |
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dc.contributor.author | Suh, M.G. | - |
dc.contributor.author | Shin, H.Y. | - |
dc.contributor.author | Jeong, E.-J. | - |
dc.contributor.author | Kim, G. | - |
dc.contributor.author | Jeong, S.B. | - |
dc.contributor.author | Ha, E.J. | - |
dc.contributor.author | Choi, S.-Y. | - |
dc.contributor.author | Moon, S.-K. | - |
dc.contributor.author | Shin, K.-S. | - |
dc.contributor.author | Yu, K.-W. | - |
dc.contributor.author | Suh, H.-J. | - |
dc.contributor.author | Kim, H. | - |
dc.date.accessioned | 2024-01-09T08:32:09Z | - |
dc.date.available | 2024-01-09T08:32:09Z | - |
dc.date.issued | 2023-04 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.issn | 1879-0003 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/70147 | - |
dc.description.abstract | Interests in the development and exploration of industrial applications of medicinal mushrooms as postbiotics have lately increased. We recently reported the potential use of Phellinus linteus mycelial-containing whole culture extract (PLME) prepared by submerged cultivation as a postbiotic that promotes immune system activation. Here, we aimed to isolate and structurally elucidate the active ingredients in PLME by activity-guided fractionation. The intestinal immunostimulatory activity was evaluated by bone marrow (BM) cell proliferation activity and related cytokine production in C3H-HeN mouse-derived Peyer's patch (PP) cells treated with polysaccharide fractions. The initially crude polysaccharide (PLME-CP) of PLME prepared using ethanol precipitation was further fractionated into four fractions (PLME-CP-0 to -III) by anion-exchange column chromatography. BM cell proliferation and cytokine production of PLME-CP-III were significantly improved compared to those of PLME-CP. PLME-CP-III was then fractionated into PLME-CP-III-1 and PLME-CP-III-2 by gel filtration chromatography. Based on the molecular weight distribution, monosaccharide, and glycosyl linkage analyses, PLME-CP-III-1 was revealed as a novel galacturonic acid-rich acidic polysaccharide and further shown to play an important role in facilitating PP-mediated intestinal immunostimulatory activity. This is the first study demonstrating the structural characteristics of a novel intestinal immune system modulating acidic polysaccharide from P. linteus mycelium-containing whole culture broth postbiotics. © 2023 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Identification of galacturonic acid-rich polysaccharide with intestinal immune system modulating activity via Peyer's patch from postbiotics of Phellinus linteus mycelial submerged culture | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2023.123685 | - |
dc.identifier.bibliographicCitation | International Journal of Biological Macromolecules, v.234 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000944268800001 | - |
dc.identifier.scopusid | 2-s2.0-85148613387 | - |
dc.citation.title | International Journal of Biological Macromolecules | - |
dc.citation.volume | 234 | - |
dc.type.docType | Article; Early Access | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Acidic polysaccharide | - |
dc.subject.keywordAuthor | Activity-guided fractionation | - |
dc.subject.keywordAuthor | Immune activity | - |
dc.subject.keywordAuthor | Medicinal mushroom | - |
dc.subject.keywordAuthor | Methylation analysis | - |
dc.subject.keywordPlus | HERICIUM-ERINACEUM MYCELIA | - |
dc.subject.keywordPlus | ACTIVITY IN-VIVO | - |
dc.subject.keywordPlus | ANTITUMOR-ACTIVITY | - |
dc.subject.keywordPlus | STRUCTURAL ELUCIDATION | - |
dc.subject.keywordPlus | IMMUNOLOGICAL ACTIVITY | - |
dc.subject.keywordPlus | FRUITING BODIES | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | BIOACTIVITIES | - |
dc.subject.keywordPlus | NIGRICANS | - |
dc.subject.keywordPlus | FEATURES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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