Genomic insight into Algoriphagus limi sp. nov. isolated from a mudflat on the Yellow Sea coast

Abstract

A Gram-negative, non-motile, and reddish-orange colored bacterium, designated CAU 1643(T), was isolated from a mudflat collected in Ganghwa Island, Republic of Korea. The bacterium was found to grow optimally at 30 degrees C, pH 9.0-9.5, and with 0%-1% (w/v) NaCl. The highest 16S rRNA gene sequence similarities to the bacterium were Algoriphagus kandeliae XY-J91(T) (97.9%), A. aquimaris F21(T) (97.1%), A. formosus XAY3209(T) (97.0%), and A. marincola DSM 16067(T) (96.2%). The DNA G + C content of the type strain was 40.35 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain CAU 1643(T) and the reference strains were below the threshold value for species demarcation. The predominant cellular fatty acids were iso-C-15:0, iso-C-17:0 3-OH, and Summed Feature 9. The major respiratory quinone was menaquinone-7. The genome showed three putative biosynthetic gene clusters that are responsible for different secondary metabolites. Moreover, CAU 1643(T) contains 72 genes that encode carbohydrate-active enzymes. Based on phenotypic, phylogenetic, and chemotaxonomic evidence, strain CAU 1643(T) represents novel species in the genus Algoriphagus, for which the name A. limi sp. nov. is proposed. The type strain is CAU 1643(T) ( = KCTC 92080(T), = MCCC 1K07150(T)). We have isolated a new Algoriphagus species, described here as A. limi sp. nov.