Thalassococcus arenae sp. nov. isolated from sea sand

Abstract

A Gram-negative, aerobic, rod-shaped bacterium, designated strain CAU 1522(T), was isolated from a sea sand sample collected from the Republic of Korea. Optimal growth of strain CAU 1522(T) ensued at 30 degrees C, pH 7.5, and 1.0% (w/v) NaCl. Strain CAU 1522(T) was affiliated to the genus Thalassococcus with high similarity to T. lentus KCTC 32084(T) (97.5%), T. profundi MCCC 1K03253(T) (96.5%), and T. halodurans JCM 13833(T) (96.1%) according to phylogenetic analysis based on 16S rRNA gene sequences. The whole genome of strain CAU 1522(T) was 3.7 Mb in length and included 7 contigs and 3599 protein-coding genes, with a G + C content of 65.4 mol%. The predominant cellular fatty acids were C-18:1 omega 6c and/or C-18:1 omega 7c (summed feature 8), with Q-10 being the sole isoprenoid quinone. The polar lipids included phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, and an unidentified lipid. These phenotypic, chemotaxonomic, and phylogenetic data support that strain CAU 1522(T) is a novel Thalassococcus species, for which the name T. arenae sp. nov. is proposed. The type strain is CAU 1522(T) (= KCTC 72545(T) = MCCC 1K04064(T)).