Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model
DC Field | Value | Language |
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dc.contributor.author | Choi, Joongwon | - |
dc.contributor.author | Jung, Tae Young | - |
dc.contributor.author | Kim, Jung Hoon | - |
dc.contributor.author | Maeng, Sejung | - |
dc.contributor.author | Kang, Su Jeong | - |
dc.contributor.author | Kim, Mirinae | - |
dc.contributor.author | Choi, Young Wook | - |
dc.contributor.author | Choi, Se Young | - |
dc.contributor.author | Kim, Sung-Hwan | - |
dc.contributor.author | Chang, In Ho | - |
dc.date.accessioned | 2024-01-18T06:30:37Z | - |
dc.date.available | 2024-01-18T06:30:37Z | - |
dc.date.issued | 2023-05 | - |
dc.identifier.issn | 2466-0493 | - |
dc.identifier.issn | 2466-054X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71069 | - |
dc.description.abstract | Purpose: We investigated the efficacy and optimal dosage of recombinant Bacillus Calmette-Guérin-dltA (rBCG-dltA) in a high-throughput 3D bio-printed bladder cancer-on-a-chip (BCOC) and orthotopic bladder cancer mouse model.Materials and Methods: We fabricated high-throughput BCOC with microfluidic systems, enabling efficient drug screening. The efficacy of rBCG-dltA was evaluated using BCOC by the cell viability assay, monocyte migration assay, and measuring cytokine levels. The anti-tumor effect was compared using the orthotopic bladder cancer mouse model.Results: The cell proliferation rates of T24 and 253J bladder cancer cell lines (mean±standard error) were measured at three days after treatment. In T24 cell line, there was significantly decreased T24 cells compared to control at rBCG 1 multiplicity of infection (MOI) and 10 MOI (30 MOI: 63.1±6.4, 10 MOI: 47.4±5.2, 1 MOI: 50.5±7.5, control: 100.0±14.5, p<0.05). In 253J cell line, a statistically significant decrease in 253J cell count compared to control and mock BCG 30 MOI (30 MOI: 11.2±1.3, 10 MOI: 22.5±2.3, 1 MOI: 39.4±4.7, Mock: 54.9±10.8, control: 100.0±5.6, p<0.05). The migration rates of THP-1 cells showed increased patterns after rBCG-dltA treatment in BCOC. The concentration of tumor necrosis factor-α and interleukin-6 after rBCG-dltA 30 MOI treatment was higher than control in T24 and 253J cell line.Conclusions: In conclusion, rBCG-dltA has the potential to have better anti-tumor activity and immunomodulatory effects than BCG. Furthermore, high-throughput BCOCs have potential to reflect the bladder cancer microenvironment. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한비뇨의학회 | - |
dc.title | Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model | - |
dc.type | Article | - |
dc.identifier.doi | 10.4111/icu.20220293 | - |
dc.identifier.bibliographicCitation | Investigative and Clinical Urology, v.64, no.3, pp 296 - 305 | - |
dc.identifier.kciid | ART002957514 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000993992000012 | - |
dc.identifier.scopusid | 2-s2.0-85160522250 | - |
dc.citation.endPage | 305 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 296 | - |
dc.citation.title | Investigative and Clinical Urology | - |
dc.citation.volume | 64 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Bioprinting | - |
dc.subject.keywordAuthor | Mycobacterium bovis | - |
dc.subject.keywordAuthor | Tumor microenvironment | - |
dc.subject.keywordPlus | BCG | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | MANAGEMENT | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | THERAPIES | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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