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Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model

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dc.contributor.authorChoi, Joongwon-
dc.contributor.authorJung, Tae Young-
dc.contributor.authorKim, Jung Hoon-
dc.contributor.authorMaeng, Sejung-
dc.contributor.authorKang, Su Jeong-
dc.contributor.authorKim, Mirinae-
dc.contributor.authorChoi, Young Wook-
dc.contributor.authorChoi, Se Young-
dc.contributor.authorKim, Sung-Hwan-
dc.contributor.authorChang, In Ho-
dc.date.accessioned2024-01-18T06:30:37Z-
dc.date.available2024-01-18T06:30:37Z-
dc.date.issued2023-05-
dc.identifier.issn2466-0493-
dc.identifier.issn2466-054X-
dc.identifier.urihttps://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71069-
dc.description.abstractPurpose: We investigated the efficacy and optimal dosage of recombinant Bacillus Calmette-Guérin-dltA (rBCG-dltA) in a high-throughput 3D bio-printed bladder cancer-on-a-chip (BCOC) and orthotopic bladder cancer mouse model.Materials and Methods: We fabricated high-throughput BCOC with microfluidic systems, enabling efficient drug screening. The efficacy of rBCG-dltA was evaluated using BCOC by the cell viability assay, monocyte migration assay, and measuring cytokine levels. The anti-tumor effect was compared using the orthotopic bladder cancer mouse model.Results: The cell proliferation rates of T24 and 253J bladder cancer cell lines (mean±standard error) were measured at three days after treatment. In T24 cell line, there was significantly decreased T24 cells compared to control at rBCG 1 multiplicity of infection (MOI) and 10 MOI (30 MOI: 63.1±6.4, 10 MOI: 47.4±5.2, 1 MOI: 50.5±7.5, control: 100.0±14.5, p<0.05). In 253J cell line, a statistically significant decrease in 253J cell count compared to control and mock BCG 30 MOI (30 MOI: 11.2±1.3, 10 MOI: 22.5±2.3, 1 MOI: 39.4±4.7, Mock: 54.9±10.8, control: 100.0±5.6, p<0.05). The migration rates of THP-1 cells showed increased patterns after rBCG-dltA treatment in BCOC. The concentration of tumor necrosis factor-α and interleukin-6 after rBCG-dltA 30 MOI treatment was higher than control in T24 and 253J cell line.Conclusions: In conclusion, rBCG-dltA has the potential to have better anti-tumor activity and immunomodulatory effects than BCG. Furthermore, high-throughput BCOCs have potential to reflect the bladder cancer microenvironment.-
dc.format.extent10-
dc.language영어-
dc.language.isoENG-
dc.publisher대한비뇨의학회-
dc.titleEfficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model-
dc.typeArticle-
dc.identifier.doi10.4111/icu.20220293-
dc.identifier.bibliographicCitationInvestigative and Clinical Urology, v.64, no.3, pp 296 - 305-
dc.identifier.kciidART002957514-
dc.description.isOpenAccessY-
dc.identifier.wosid000993992000012-
dc.identifier.scopusid2-s2.0-85160522250-
dc.citation.endPage305-
dc.citation.number3-
dc.citation.startPage296-
dc.citation.titleInvestigative and Clinical Urology-
dc.citation.volume64-
dc.type.docTypeArticle-
dc.publisher.location대한민국-
dc.subject.keywordAuthorBioprinting-
dc.subject.keywordAuthorMycobacterium bovis-
dc.subject.keywordAuthorTumor microenvironment-
dc.subject.keywordPlusBCG-
dc.subject.keywordPlusASSOCIATION-
dc.subject.keywordPlusMANAGEMENT-
dc.subject.keywordPlusCARCINOMA-
dc.subject.keywordPlusTHERAPIES-
dc.subject.keywordPlusCELLS-
dc.relation.journalResearchAreaUrology & Nephrology-
dc.relation.journalWebOfScienceCategoryUrology & Nephrology-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
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