Neuroprotective effects of tumor necrosis factor-α inhibitor on rat hippocampal organotypic slice cultures treated with the 1-42 β-amyloid
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 허재혁 | - |
dc.contributor.author | 현재경 | - |
dc.contributor.author | 윤영철 | - |
dc.date.accessioned | 2024-01-31T04:00:28Z | - |
dc.date.available | 2024-01-31T04:00:28Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 2799-4090 | - |
dc.identifier.issn | 2799-4104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71605 | - |
dc.description.abstract | Background: Alzheimer disease (AD) is a chronic degenerative neuronal disease. Tumor necrosis factor (TNF) inhibitors involved in neuroinflammation in AD pathogenesis can be a potential treatment strategy for AD. Therefore, we performed organotypic hippocampal slice culture to investigate neuroprotective effects by TNF-α inhibitor from β-amyloid (Aβ)-induced neurotoxicity. Methods: We cultured the hippocampus of a 7 days postnatal Sprague-Dawley rats with 450 µm thick slices. The tissue slices had been exposed with 100 µM Aβ at an interval of 2 days since 14 days in vitro. Following co-treatment of the tissue slice with 100 µg/ml adalimumab and Aβ, we evaluated adalimumab effect on Aβ induced neurotoxicity by morphological observation of the hippocampus slice cultures with 1 µg/ml propidium iodide (PI) and measuring the expression levels of NeuN, Bcl2, and tau proteins. Results: Aβ (100 µM) induced increase of PI-positive fluorescence in the hippocampal slice. The adalimumab as a TNF-α inhibitor showed protective effects for Aβ-induced neurotoxicity, and reduced cell death of the CA1 region in hippocampal slice. The expression of NeuN and Bcl2 proteins was significantly decreased by 100 µM Aβ, and increased by co-treatment of 100 µg/ml adalimumab. Phosphorylation of tau protein increased by 100 µM Aβ and decreased by co-treatment of 100 µg/ml adalimumab. Conclusions: Adalimumab reduced the Aβ-induced neurotoxicity of the hippocampal slice culture, inhibited expression of NeuN, Bcl2 proteins and increasing of phophorylated tau protein. These findings would be useful as baseline data in the field of AD research and development of noble drugs for neurodegenerative diseases. | - |
dc.format.extent | 5 | - |
dc.publisher | Korean Society of Geriatric Neurology | - |
dc.title | Neuroprotective effects of tumor necrosis factor-α inhibitor on rat hippocampal organotypic slice cultures treated with the 1-42 β-amyloid | - |
dc.title.alternative | 쥐 해마 절편배양에서 β-아밀로이드 1-42분획 독성에 대한 종양괴사인자-α 억제제의 세포 보호 효과 | - |
dc.type | Article | - |
dc.identifier.doi | 10.53991/jgn.2021.00031 | - |
dc.identifier.bibliographicCitation | Journal of Geriatric Neurology, v.1, no.1, pp 33 - 37 | - |
dc.description.isOpenAccess | Y | - |
dc.citation.endPage | 37 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 33 | - |
dc.citation.title | Journal of Geriatric Neurology | - |
dc.citation.volume | 1 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Alzheimer disease | - |
dc.subject.keywordAuthor | Hippocampal slice culture | - |
dc.subject.keywordAuthor | Beta-amyloid | - |
dc.subject.keywordAuthor | Tumor necrosis factor inhibitors | - |
dc.description.journalRegisteredClass | domestic | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.