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C-Glycoside-Metabolizing Human Gut Bacterium, Dorea sp. MRG-IFC3

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dc.contributor.authorMi Huynh Thi Ngoc-
dc.contributor.authorChaiyasarn Santipap-
dc.contributor.authorKim Heji-
dc.contributor.authorHan Jaehong-
dc.date.accessioned2024-02-13T01:31:31Z-
dc.date.available2024-02-13T01:31:31Z-
dc.date.issued2023-12-
dc.identifier.issn1017-7825-
dc.identifier.issn1738-8872-
dc.identifier.urihttps://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71911-
dc.description.abstractBiochemical gut metabolism of dietary bioactive compounds is of great significance in elucidating health-related issues at the molecular level. In this study, a human gut bacterium cleaving C-C glycosidic bond was screened from puerarin conversion to daidzein, and a new, gram-positive Cglycoside-deglycosylating strain, Dorea sp. MRG-IFC3, was isolated from human fecal sample under anaerobic conditions. Though MRG-IFC3 biotransformed isoflavone C-glycoside, it could not metabolize other C-glycosides, such as vitexin, bergenin, and aloin. As evident from the production of the corresponding aglycons from various 7-O-glucosides, MRG-IFC3 strain also showed 7-Oglycoside cleavage activity; however, flavone 3-O-glucoside icariside II was not metabolized. In addition, for mechanism study, C-glycosyl bond cleavage of puerarin by MRG-IFC3 strain was performed in D2O GAM medium. The complete deuterium enrichment on C-8 position of daidzein was confirmed by 1 H NMR spectroscopy, and the result clearly proved for the first time that daidzein is produced from puerarin. Two possible reaction intermediates, the quinoids and 8-dehydrodaidzein anion, were proposed for the production of daidzein-8d. These results will provide the basis for the mechanism study of stable C-glycosidic bond cleavage at the molecular level.-
dc.format.extent9-
dc.language영어-
dc.language.isoENG-
dc.publisher한국미생물·생명공학회-
dc.titleC-Glycoside-Metabolizing Human Gut Bacterium, Dorea sp. MRG-IFC3-
dc.typeArticle-
dc.identifier.doi10.4014/jmb.2308.08021-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, v.33, no.12, pp 1606 - 1614-
dc.identifier.kciidART003035872-
dc.description.isOpenAccessN-
dc.identifier.wosid001166324900007-
dc.identifier.scopusid2-s2.0-85181396380-
dc.citation.endPage1614-
dc.citation.number12-
dc.citation.startPage1606-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.volume33-
dc.type.docTypeArticle-
dc.publisher.location대한민국-
dc.subject.keywordAuthorC-C bond cleavage-
dc.subject.keywordAuthorC-glycoside-
dc.subject.keywordAuthorDorea sp. MRG-IFC3-
dc.subject.keywordAuthorgut metabolism-
dc.subject.keywordAuthorpuerarin-
dc.subject.keywordPlusHUMAN INTESTINAL BACTERIUM-
dc.subject.keywordPlusDEGLYCOSYLATION-
dc.subject.keywordPlusNORATHYRIOL-
dc.subject.keywordPlusMANGIFERIN-
dc.subject.keywordPlusPUERARIN-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaMicrobiology-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryMicrobiology-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
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