C-Glycoside-Metabolizing Human Gut Bacterium, Dorea sp. MRG-IFC3
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mi Huynh Thi Ngoc | - |
dc.contributor.author | Chaiyasarn Santipap | - |
dc.contributor.author | Kim Heji | - |
dc.contributor.author | Han Jaehong | - |
dc.date.accessioned | 2024-02-13T01:31:31Z | - |
dc.date.available | 2024-02-13T01:31:31Z | - |
dc.date.issued | 2023-12 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/71911 | - |
dc.description.abstract | Biochemical gut metabolism of dietary bioactive compounds is of great significance in elucidating health-related issues at the molecular level. In this study, a human gut bacterium cleaving C-C glycosidic bond was screened from puerarin conversion to daidzein, and a new, gram-positive Cglycoside-deglycosylating strain, Dorea sp. MRG-IFC3, was isolated from human fecal sample under anaerobic conditions. Though MRG-IFC3 biotransformed isoflavone C-glycoside, it could not metabolize other C-glycosides, such as vitexin, bergenin, and aloin. As evident from the production of the corresponding aglycons from various 7-O-glucosides, MRG-IFC3 strain also showed 7-Oglycoside cleavage activity; however, flavone 3-O-glucoside icariside II was not metabolized. In addition, for mechanism study, C-glycosyl bond cleavage of puerarin by MRG-IFC3 strain was performed in D2O GAM medium. The complete deuterium enrichment on C-8 position of daidzein was confirmed by 1 H NMR spectroscopy, and the result clearly proved for the first time that daidzein is produced from puerarin. Two possible reaction intermediates, the quinoids and 8-dehydrodaidzein anion, were proposed for the production of daidzein-8d. These results will provide the basis for the mechanism study of stable C-glycosidic bond cleavage at the molecular level. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | C-Glycoside-Metabolizing Human Gut Bacterium, Dorea sp. MRG-IFC3 | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.2308.08021 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.33, no.12, pp 1606 - 1614 | - |
dc.identifier.kciid | ART003035872 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 001166324900007 | - |
dc.identifier.scopusid | 2-s2.0-85181396380 | - |
dc.citation.endPage | 1614 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1606 | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 33 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | C-C bond cleavage | - |
dc.subject.keywordAuthor | C-glycoside | - |
dc.subject.keywordAuthor | Dorea sp. MRG-IFC3 | - |
dc.subject.keywordAuthor | gut metabolism | - |
dc.subject.keywordAuthor | puerarin | - |
dc.subject.keywordPlus | HUMAN INTESTINAL BACTERIUM | - |
dc.subject.keywordPlus | DEGLYCOSYLATION | - |
dc.subject.keywordPlus | NORATHYRIOL | - |
dc.subject.keywordPlus | MANGIFERIN | - |
dc.subject.keywordPlus | PUERARIN | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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