Circulating autoreactive proteinase 3<SUP>+</SUP> B cells and tolerance checkpoints in ANCA-associated vasculitisopen access
- Authors
- Berti, Alvise; Hillion, Sophie; Hummel, Amber M.; Son, Young Min; Chriti, Nedra; Peikert, Tobias; Carmona, Eva M.; Abdulahad, Wayel H.; Heeringa, Peter; Harris, Kristina M.; St Clair, E. William; Brunetta, Paul; Fervenza, Fernando C.; Langford, Carol A.; Kallenberg, Cees G. M.; Merkel, Peter A.; Monach, Paul A.; Seo, Philip; Spiera, Robert F.; Stone, John H.; Grandi, Guido; Sun, Jie; Pers, Jacques-Olivier; Specks, Ulrich; Cornec, Divi
- Issue Date
- Nov-2021
- Publisher
- AMER SOC CLINICAL INVESTIGATION INC
- Citation
- JCI INSIGHT, v.6, no.22
- Journal Title
- JCI INSIGHT
- Volume
- 6
- Number
- 22
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/72077
- DOI
- 10.1172/jci.insight.150999
- ISSN
- 2324-7703
2379-3708
- Abstract
- BACKGROUND. Little is known about the autoreactive B cells in antineutrophil cytoplasmic antibody-associated (ANCA-associated) vasculitis (AAV). We aimed to investigate tolerance checkpoints of circulating antigen-specific proteinase 3-reactive (PR3(+)) B cells. METHODS. Multicolor flow cytometry in combination with bioinformatics and functional in vitro studies were performed on baseline samples of PBMCs from 154 well-characterized participants of the RAVE trial (NCT00104299) with severely active PR3-AAV and myeloperoxidase-AAV (MPO-AAV) and 27 healthy controls (HCs). Clinical data and outcomes from the trial were correlated with PR3(+) B cells (total and subsets). RESULTS. The frequency of PR3(+) B cells among circulating B cells was higher in participants with PR3AAV (4.77% median [IQR, 3.98%-6.01%]) than in participants with MPO-AAV (3.16% median [IQR, 2.51%-5.22%]) and participants with AAV compared with HCs (1.67% median [IQR,1.27%-2.16%], P< 0.001 for all comparisons), implying a defective central tolerance checkpoint in patients with AAV. Only PBMCs from participants with PR3-AAV contained PR3(+) B cells capable of secreting PR3-ANCA IgG in vitro, proving they were functionally distinct from those of participants with MPO-AAV and HCs. Unsupervised clustering identified subtle subsets of atypical autoreactive PR3(+) memory B cells accumulating through the maturation process in patients with PR3-AAV. PR3(+) B cells were enriched in the memory B cell compartment of participants with PR3-AAV and were associated with higher serum CXCL13 levels. suggesting an increased germinal center activity. PR3(+) B cells correlated with systemic inflammation (C-reactive protein and erythrocyte sedimentation rate. P < 0.05) and complete remission (P< 0.001). CONCLUSION. This study suggests the presence of defective central antigen-independent and peripheral antigen-dependent checkpoints in patients with PR3-AAV, elucidating the selection process of autoreactive B cells.
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