Single-cell mass cytometry on peripheral blood identifies immune cell subsets associated with primary biliary cholangitis
DC Field | Value | Language |
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dc.contributor.author | Jang, Jin Sung | - |
dc.contributor.author | Juran, Brian D. | - |
dc.contributor.author | Cunningham, Kevin Y. | - |
dc.contributor.author | Gupta, Vinod K. | - |
dc.contributor.author | Son, Young Min | - |
dc.contributor.author | Yang, Ju Dong | - |
dc.contributor.author | Ali, Ahmad H. | - |
dc.contributor.author | Enninga, Elizabeth Ann L. | - |
dc.contributor.author | Sung, Jaeyun | - |
dc.contributor.author | Lazaridis, Konstantinos N. | - |
dc.date.accessioned | 2024-02-19T01:30:24Z | - |
dc.date.available | 2024-02-19T01:30:24Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/72084 | - |
dc.description.abstract | The relationship between primary biliary cholangitis (PBC), a chronic cholestatic autoimmune liver disease, and the peripheral immune system remains to be fully understood. Herein, we performed the first mass cytometry (CyTOF)-based, immunophenotyping analysis of the peripheral immune system in PBC at single-cell resolution. CyTOF was performed on peripheral blood mononuclear cells (PBMCs) from PBC patients (n=33) and age-/sex-matched healthy controls (n=33) to obtain immune cell abundance and marker expression profiles. Hierarchical clustering methods were applied to identify immune cell types and subsets significantly associated with PBC. Subsets of gamma-delta T cells (CD3(+)TCRgd(+)), CD8(+) T cells (CD3(+)CD8(+)CD161(+)PD1(+)), and memory B cells (CD3(-)CD19(+)CD20(+)CD24(+)CD27(+)) were found to have lower abundance in PBC than in control. In contrast, higher abundance of subsets of monocytes and naive B cells were observed in PBC compared to control. Furthermore, several naive B cell (CD3(-)CD19(+)CD20(+)CD24(-)CD27(-)) subsets were significantly higher in PBC patients with cirrhosis (indicative of late-stage disease) than in those without cirrhosis. Alternatively, subsets of memory B cells were lower in abundance in cirrhotic relative to non-cirrhotic PBC patients. Future immunophenotyping investigations could lead to better understanding of PBC pathogenesis and progression, and also to the discovery of novel biomarkers and treatment strategies. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Single-cell mass cytometry on peripheral blood identifies immune cell subsets associated with primary biliary cholangitis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41598-020-69358-4 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.10, no.1 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000556398500007 | - |
dc.identifier.scopusid | 2-s2.0-85088642045 | - |
dc.citation.number | 1 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 10 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | REGULATORY B-CELLS | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | CIRRHOSIS | - |
dc.subject.keywordPlus | VISUALIZATION | - |
dc.subject.keywordPlus | MONOCYTES | - |
dc.subject.keywordPlus | ELEVATION | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | LIVERS | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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