A Bacterial Metabolite, Compound K, Induces Programmed Necrosis in MCF-7 Cells <i>via</i> GSK3β
DC Field | Value | Language |
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dc.contributor.author | Kwak, Chae Won | - |
dc.contributor.author | Son, Young Min | - |
dc.contributor.author | Gu, Min Jeong | - |
dc.contributor.author | Kim, Girak | - |
dc.contributor.author | Lee, In Kyu | - |
dc.contributor.author | Kye, Yoon Chul | - |
dc.contributor.author | Kim, Han Wool | - |
dc.contributor.author | Song, Ki-Duk | - |
dc.contributor.author | Chu, Hyuk | - |
dc.contributor.author | Park, Byung-Chul | - |
dc.contributor.author | Lee, Hak-Kyo | - |
dc.contributor.author | Yang, Deok-Chun | - |
dc.contributor.author | Sprent, Jonathan | - |
dc.contributor.author | Yun, Cheol-Heui | - |
dc.date.accessioned | 2024-02-19T02:30:30Z | - |
dc.date.available | 2024-02-19T02:30:30Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/72105 | - |
dc.description.abstract | Ginsenosides, the major active component of ginseng, are traditionally used to treat various diseases, including cancer, inflammation, and obesity. Among these, compound K (CK), an intestinal bacterial metabolite of the ginsenosides Rb-1, Rb-2, and Rc from Bacteroides JY-6, is reported to inhibit cancer cell growth by inducing cell-cycle arrest or cell death, including apoptosis and necrosis. However, the precise effect of CK on breast cancer cells remains unclear. MCF-7 cells were treated with CK (0-70 mu M) for 24 or 48 h. Cell proliferation and death were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Changes in downstream signaling molecules involved in cell death, including glycogen synthase kinase 3 beta (GSK3 beta), GSK3 beta, beta-catenin, and cyclin D1, were analyzed by western blot assay. To block GSK3 beta signaling, MCF-7 cells were pretreated with GSK3 beta inhibitors 1. h prior to CK treatment. Cell death and the expression of beta-catenin and cyclin D1 were then examined. CK dose- and time-dependently inhibited MCF-7 cell proliferation. Interestingly, CK induced programmed necrosis, but not apoptosis, via the GSK3 beta signaling pathway in MCF-7 cells. CK inhibited GSK3 beta phosphorylation, thereby suppressing the expression of beta-catenin and cyclin D1. Our results suggest that CK induces programmed necrosis in MCF-7 breast cancer cells via the GSK3 beta signaling pathway. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | A Bacterial Metabolite, Compound K, Induces Programmed Necrosis in MCF-7 Cells <i>via</i> GSK3β | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.1505.05057 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.7, pp 1170 - 1176 | - |
dc.identifier.kciid | ART002014190 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000358701800024 | - |
dc.identifier.scopusid | 2-s2.0-84937390880 | - |
dc.citation.endPage | 1176 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1170 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 25 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Compound K | - |
dc.subject.keywordAuthor | ginsenoside | - |
dc.subject.keywordAuthor | programmed necrosis | - |
dc.subject.keywordAuthor | breast cancer cells | - |
dc.subject.keywordAuthor | MCF-7 | - |
dc.subject.keywordAuthor | GSK3 beta | - |
dc.subject.keywordPlus | SYNTHASE KINASE 3-BETA | - |
dc.subject.keywordPlus | POLY(ADP-RIBOSE) POLYMERASE-1 | - |
dc.subject.keywordPlus | GINSENOSIDE METABOLITE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GSK-3-BETA | - |
dc.subject.keywordPlus | PROTEOLYSIS | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | SAPONIN | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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