Castasterone Can be Biosynthesized from 28-homodolichosterone in Arabidopsis thaliana
DC Field | Value | Language |
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dc.contributor.author | Kim, Sunyoung | - |
dc.contributor.author | Moon, Jinyoung | - |
dc.contributor.author | Roh, Jeehee | - |
dc.contributor.author | Kim, Seong-Ki | - |
dc.date.available | 2019-01-22T12:34:58Z | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 1226-9239 | - |
dc.identifier.issn | 1867-0725 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/721 | - |
dc.description.abstract | We recently demonstrated the biosynthesis of 24-ethylidene brassinosteroids in Arabidopsis thaliana. To determine the physiological role of biosynthesis of 24-ethylidene brassinosteroids, metabolism of 28-homodolichosterone as the end product of 24-ethylidene brassinosteroids biosynthesis was examined by a crude enzyme solution prepared from A. thaliana. In wild-type plants, dolichosterone and castasterone were identified as enzyme products on GC-MS analysis. In a mutant where DWARF1 was overexpressed (35S-DWF1), the conversion rate of 28-homodolichosterone to castasterone was significantly increased. These results indicate that conversion of 28-homodolichosterone to castasterone is mediated by dolichosterone in Arabidopsis. In the root growth assay, inhibitory activity was enhanced in the order of castasterone > dolichosterone > 28-homodolichosterone, demonstrating that conversion of 28-homodolichosterone to castasterone via dolichosterone is a biosynthetic reaction that increases BR activity in Arabidopsis. Compared to Arabidopsis grown under dark conditions, light-grown Arabidopsis showed up-regulated DWARF1 expression, resulting in an increased conversion rate of 28-homodolichosterone to castasterone, suggesting that light is an important regulatory factor for the biosynthetic connection of 24-ethylidene brassinosteroids and 24-methyl brassinosteroids in A. thaliana. Consequently, 24-ethylidene brassinosteroids biosynthesis to generate 28-homodolichosterone is a lightregulated alternative route for synthesis of the biologically-active BRs, castasterone and brassinolide in Arabidopsis plants. | - |
dc.format.extent | 6 | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Castasterone Can be Biosynthesized from 28-homodolichosterone in Arabidopsis thaliana | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12374-018-0194-4 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PLANT BIOLOGY, v.61, no.5, pp 330 - 335 | - |
dc.identifier.kciid | ART002466206 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000446182500007 | - |
dc.identifier.scopusid | 2-s2.0-85054163682 | - |
dc.citation.endPage | 335 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 330 | - |
dc.citation.title | JOURNAL OF PLANT BIOLOGY | - |
dc.citation.volume | 61 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Arabidopsis thaliana | - |
dc.subject.keywordAuthor | Biosynthesis of 24-ethylidene brassinosteroids | - |
dc.subject.keywordAuthor | Biosynthetic connection of C-28-Brassinosteroids and C-29-brassinosteroids | - |
dc.subject.keywordAuthor | Metabolism of 28-homodolichosterone | - |
dc.subject.keywordAuthor | Synthesis of castasterone | - |
dc.subject.keywordPlus | BRASSINOSTEROID BIOSYNTHESIS | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | BRASSINOLIDE | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | HORMONES | - |
dc.subject.keywordPlus | GROWTH | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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