The triazole fungicide metconazole inhibits the homodimerization of human androgen receptors to suppress androgen-induced transcriptional activation
- Authors
- Jung, Da-Woon; Jeong, Da-Hyun; Kim, Uk-Jin; Lee, Hee-Seok
- Issue Date
- Jun-2023
- Publisher
- Elsevier Ireland Ltd
- Keywords
- Androgen receptor; Endocrine-disrupting effect; Genomic pathway; Metconazole
- Citation
- Chemico-Biological Interactions, v.378
- Journal Title
- Chemico-Biological Interactions
- Volume
- 378
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/72563
- DOI
- 10.1016/j.cbi.2023.110489
- ISSN
- 0009-2797
1872-7786
- Abstract
- We assessed the mechanism of human androgen receptor-mediated endocrine-disrupting effect by a triazole fungicide, metconazole in this study. The internationally validated stably transfected transactivation (STTA) in vitro assay, which was established for determination of a human androgen receptor (AR) agonist/antagonist by using 22Rv1/MMTV_GR-KO cell line, alongside an in vitro reporter-gene assay to confirm AR homodimerization was used. The STTA in vitro assay results showed that metconazole is a true AR antagonist. Furthermore, the results from the in vitro reporter-gene assay and western blotting showed that metconazole blocks the nuclear transfer of cytoplasmic AR proteins by suppressing the homodimerization of AR. These results suggest that metconazole can be considered to have an AR-mediated endocrine-disrupting effect. Additionally, the evidence from this study might help identify the endocrine-disrupting mechanism of triazole fungicides containing a phenyl ring. © 2023 Elsevier B.V.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.