Single-Nucleotide Microbial Genome Editing Using CRISPR-Cas12a
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Ho Joung | - |
dc.contributor.author | Lee, Sang Jun | - |
dc.date.accessioned | 2024-04-04T01:00:27Z | - |
dc.date.available | 2024-04-04T01:00:27Z | - |
dc.date.issued | 2024-03 | - |
dc.identifier.issn | 1940-6029 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73132 | - |
dc.description.abstract | Microbial genome editing can be achieved by donor DNA-directed mutagenesis and CRISPR-Cas12a-mediated negative selection. Single-nucleotide-level genome editing enables the manipulation of microbial cells exactly as designed. Here, we describe single-nucleotide substitutions/indels in the target DNA of E. coli genome using a mutagenic DNA oligonucleotide donor and truncated crRNA/Cas12a system. The maximal truncation of nucleotides at the 3'-end of the crRNA enables Cas12a-mediated single-nucleotide-level precise editing at galK targets in the genome of E. coli. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Humana Press Inc. | - |
dc.title | Single-Nucleotide Microbial Genome Editing Using CRISPR-Cas12a | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/978-1-0716-3658-9_9 | - |
dc.identifier.bibliographicCitation | Methods in molecular biology (Clifton, N.J.), v.2760, pp 147 - 155 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85187780845 | - |
dc.citation.endPage | 155 | - |
dc.citation.startPage | 147 | - |
dc.citation.title | Methods in molecular biology (Clifton, N.J.) | - |
dc.citation.volume | 2760 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | 3′-truncated crRNA | - |
dc.subject.keywordAuthor | Precise genome editing | - |
dc.subject.keywordAuthor | Single-base | - |
dc.subject.keywordAuthor | Cas12a | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.