Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Mi Kyung | - |
dc.contributor.author | Kim, Hye Ryoun | - |
dc.date.accessioned | 2024-04-13T01:00:27Z | - |
dc.date.available | 2024-04-13T01:00:27Z | - |
dc.date.issued | 2006-06 | - |
dc.identifier.issn | 1598-6535 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73211 | - |
dc.description.abstract | BACKGROUND: Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and a researcher-friendly gel electrophoresis-based PCR method for the quantification of mRNA, and the results were compared with those obtained by real-time PCR. METHODS: We compared the results of relative quantification for MMP-1 measured by real-time PCR and by ethidium bromide stained-agarose gel electrophoresis after end-point PCR. RESULTS: There was significant but very weak correlation between real-time PCR and end-point PCR for relative quantification of MMP-1 (r=0.16, P<0.01). CONCLUSIONS: Our results suggest that the use of the gel electrophoresis-based end-point PCR is inappropriate for quantifying mRNA. Therefore, in order to confirm the result of relative quantification by end-point PCR, the newly established real-time PCR method or northern hybridization should be applied. | - |
dc.format.extent | 6 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.title | Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification | - |
dc.title.alternative | 실시간 중합효소연쇄반응검사와 아가로오스겔 전기영동에 의한 중합효소연쇄반응물의 정량검사 비교 | - |
dc.type | Article | - |
dc.identifier.doi | 10.3343/kjlm.2006.26.3.217 | - |
dc.identifier.bibliographicCitation | The Korean journal of laboratory medicine, v.26, no.3, pp 217 - 222 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 222 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 217 | - |
dc.citation.title | The Korean journal of laboratory medicine | - |
dc.citation.volume | 26 | - |
dc.type.docType | English Abstract;Journal Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | mRNA quantification | - |
dc.subject.keywordAuthor | Real-time PCR | - |
dc.subject.keywordAuthor | End-point PCR | - |
dc.subject.keywordAuthor | Agarose gel electrophoresis | - |
dc.description.journalRegisteredClass | domestic | - |
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