Nanozyme-assisted molecularly imprinted polymer-based indirect competitive ELISA for the detection of marine biotoxin
DC Field | Value | Language |
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dc.contributor.author | Cho, Chae Hwan | - |
dc.contributor.author | Kim, Ji Hong | - |
dc.contributor.author | Padalkar, Navnath S | - |
dc.contributor.author | Reddy, Y Veera Manohara | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Park, Jinyoung | - |
dc.contributor.author | Park, Jong Pil | - |
dc.date.accessioned | 2024-04-19T05:30:28Z | - |
dc.date.available | 2024-04-19T05:30:28Z | - |
dc.date.issued | 2024-07 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73326 | - |
dc.description.abstract | Saxitoxin (STX), which is produced by certain dinoflagellate species, is a type of paralytic shellfish poisoning toxin that poses a serious threat to human health and the environment. Therefore, developing a technology for the convenient and cost-effective detection of STX is imperative. In this study, we developed an affinity peptide-imprinted polymer-based indirect competitive ELISA (ic-ELISA) without using enzyme-toxin conjugates. AuNP/Co3O4@Mg/Al cLDH was synthesized by calcining AuNP/ZIF-67@Mg/Al LDH, which was obtained by combining AuNPs, ZIF-67, and flower-like Mg/Al LDH. This synthesized nanozyme exhibited high catalytic activity (Km=0.24 mM for TMB and 132.5 mM for H2O2). The affinity peptide-imprinted polymer (MIP) was imprinted with an STX-specific template peptide (STX MIP) on a multi-well microplate and then reacted with an STX-specific signal peptide (STX SP). The interaction between the STX SP and MIP was detected using a streptavidin-coated nanozyme (SA-AuNP/Co3O4@Mg/Al cLDH). The developed MIP-based ic-ELISA exhibited excellent selectivity and sensitivity, with a limit of detection of 3.17 ng/mL (equivalent: 0.317 μg/g). Furthermore, the system was validated using a commercial ELISA kit and mussel tissue samples, and it demonstrated a high STX recovery with a low coefficient of variation. These results imply that the developed ic-ELISA can be used to detect STX in real samples. Copyright © 2024 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Nanozyme-assisted molecularly imprinted polymer-based indirect competitive ELISA for the detection of marine biotoxin | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2024.116269 | - |
dc.identifier.bibliographicCitation | Biosensors & bioelectronics, v.255 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 001226296800001 | - |
dc.identifier.scopusid | 2-s2.0-85189528974 | - |
dc.citation.title | Biosensors & bioelectronics | - |
dc.citation.volume | 255 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Affinity peptide | - |
dc.subject.keywordAuthor | ELISA | - |
dc.subject.keywordAuthor | Marine toxin | - |
dc.subject.keywordAuthor | Molecularly imprinted polymer | - |
dc.subject.keywordAuthor | MIP | - |
dc.subject.keywordAuthor | Nanozyme | - |
dc.subject.keywordPlus | PEROXIDASE-LIKE ACTIVITY | - |
dc.subject.keywordPlus | PSEUDO-ELISA | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | AFFINITY | - |
dc.subject.keywordPlus | DISPLAY | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | ACID) | - |
dc.subject.keywordPlus | WATER | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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