Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins
DC Field | Value | Language |
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dc.contributor.author | Lee, Eunsang | - |
dc.contributor.author | Kim, Donghee | - |
dc.contributor.author | Song, Yo Han | - |
dc.contributor.author | Shin, Kyujin | - |
dc.contributor.author | Song, Sanggeun | - |
dc.contributor.author | Lee, Minho | - |
dc.contributor.author | Goh, Yeongchang | - |
dc.contributor.author | Lim, Mi Hee | - |
dc.contributor.author | Kim, Ji-Hyun | - |
dc.contributor.author | Sung, Jaeyoung | - |
dc.contributor.author | Lee, Kang Taek | - |
dc.date.accessioned | 2024-05-21T05:30:36Z | - |
dc.date.available | 2024-05-21T05:30:36Z | - |
dc.date.issued | 2024-05 | - |
dc.identifier.issn | 2832-3637 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73854 | - |
dc.description.abstract | Synaptic vesicle transport by motor proteins along microtubules is a crucially active process underlying neuronal communication. It is known that microtubules are destabilized by tau-hyperphosphorylation, which causes tau proteins to detach from microtubules and form neurofibril tangles. However, how tau-phosphorylation affects the transport dynamics of motor proteins on the microtubule remains unknown. Here, we discover that the long-distance unidirectional motion of vesicle-motor protein multiplexes (VMPMs) in living cells is suppressed under tau-hyperphosphorylation, with the consequent loss of fast vesicle-transport along the microtubule. The VMPMs in hyperphosphorylated cells exhibit seemingly bidirectional random motion, with dynamic properties far different from those of VMPM motion in normal cells. We establish a parsimonious physicochemical model of VMPM’s active motion that provides a unified, quantitative explanation and predictions for our experimental results. Our analysis reveals that, under hyperphosphorylation conditions, motor protein multiplexes have both static and dynamic motility fluctuations. The loss of fast vesicle-transport along the microtubule can be a mechanism of neurodegenerative disorders associated with tau-hyperphosphorylation. © 2024 The Authors. Co-published by Nanjing University and American Chemical Society | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/cbmi.4c00016 | - |
dc.identifier.bibliographicCitation | Chemical and Biomedical Imaging, v.2, no.5, pp 362 - 373 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85191799354 | - |
dc.citation.endPage | 373 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 362 | - |
dc.citation.title | Chemical and Biomedical Imaging | - |
dc.citation.volume | 2 | - |
dc.type.docType | Article in press | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | microtubule | - |
dc.subject.keywordAuthor | motor protein | - |
dc.subject.keywordAuthor | tau aggregation | - |
dc.subject.keywordAuthor | transport dynamics | - |
dc.subject.keywordAuthor | upconverting nanoparticle | - |
dc.description.journalRegisteredClass | scopus | - |
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