Plant-Derived Anti-Human Epidermal Growth Factor Receptor 2 Antibody Suppresses Trastuzumab-Resistant Breast Cancer with Enhanced Nanoscale Binding
DC Field | Value | Language |
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dc.contributor.author | Park, Chanyong | - |
dc.contributor.author | Kim, Kibum | - |
dc.contributor.author | Kim, Yerin | - |
dc.contributor.author | Zhu, Rong | - |
dc.contributor.author | Hain, Lisa | - |
dc.contributor.author | Seferovic, Hannah | - |
dc.contributor.author | Kim, Min-Hyeok | - |
dc.contributor.author | Woo, Hyun Joo | - |
dc.contributor.author | Hwang, Hyunju | - |
dc.contributor.author | Lee, Seung Ho | - |
dc.contributor.author | Kim, Sangmin | - |
dc.contributor.author | Lee, Jeong Eon | - |
dc.contributor.author | Hinterdorfer, Peter | - |
dc.contributor.author | Ko, Kisung | - |
dc.contributor.author | Park, Sungsu | - |
dc.contributor.author | Oh, Yoo Jin | - |
dc.date.accessioned | 2024-06-14T05:30:30Z | - |
dc.date.available | 2024-06-14T05:30:30Z | - |
dc.date.issued | 2024-05 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.issn | 1936-086X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74178 | - |
dc.description.abstract | Traditional monoclonal antibodies such as Trastuzumab encounter limitations when treating Human Epidermal Growth Factor Receptor 2 (HER2)-positive breast cancer, particularly in cases that develop resistance. This study introduces plant-derived anti-HER2 variable fragments of camelid heavy chain domain (VHH) fragment crystallizable region (Fc) KEDL(K) antibody as a potent alternative for overcoming these limitations. A variety of biophysical techniques, in vitro assays, and in vivo experiments uncover the antibody’s nanoscale binding dynamics with transmembrane HER2 on living cells. Single-molecule force spectroscopy reveals the rapid formation of two robust bonds, exhibiting approximately 50 pN force resistance and bond lifetimes in the second range. The antibody demonstrates a specific affinity for HER2-positive breast cancer cells, including those that are Trastuzumab-resistant. Moreover, in immune-deficient mice, the plant-derived anti-HER2 VHH-FcK antibody exhibits superior antitumor activity, especially against tumors that are resistant to Trastuzumab. These findings underscore the plant-derived antibody’s potential as an impactful immunotherapeutic strategy for treating Trastuzumab-resistant HER2-positive breast cancer. © 2024 The Authors. Published by American Chemical Society. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Plant-Derived Anti-Human Epidermal Growth Factor Receptor 2 Antibody Suppresses Trastuzumab-Resistant Breast Cancer with Enhanced Nanoscale Binding | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acsnano.4c00360 | - |
dc.identifier.bibliographicCitation | ACS Nano | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 001227979100001 | - |
dc.identifier.scopusid | 2-s2.0-85193754423 | - |
dc.citation.title | ACS Nano | - |
dc.type.docType | Article; Early Access | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | DYNAMIC FORCE SPECTROSCOPY | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | HER2 | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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