Single-cell RNA sequencing reveals the heterogeneity of adipose tissue-derived mesenchymal stem cells under chondrogenic induction
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chun, Jeewan | - |
dc.contributor.author | Moon, Ji-Hoi | - |
dc.contributor.author | Kwack, Kyu Hwan | - |
dc.contributor.author | Jang, Eun-Young | - |
dc.contributor.author | Lee, Saebyeol | - |
dc.contributor.author | Kim, Hak Kyun | - |
dc.contributor.author | Lee, Jae-Hyung | - |
dc.date.accessioned | 2024-06-17T03:00:26Z | - |
dc.date.available | 2024-06-17T03:00:26Z | - |
dc.date.issued | 2024-05 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.issn | 1976-670X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74242 | - |
dc.description.abstract | This study investigated how adipose tissue-derived mesenchymal stem cells (AT-MSCs) respond to chondrogenic induction using droplet-based single-cell RNA sequencing (scRNA-seq). We analyzed 37,219 high-quality transcripts from control cells and cells induced for 1 week (1W) and 2 weeks (2W). Four distinct cell clusters (0-3), undetectable by bulk analysis, exhibited varying proportions. Cluster 1 dominated in control and 1W cells, whereas clusters (3, 2, and 0) exclusively dominated in control, 1W, and 2W cells, respectively. Furthermore, heterogeneous chondrogenic markers expression within clusters emerged. Gene ontology (GO) enrichment analysis of differentially expressed genes unveiled cluster-specific variations in key biological processes (BP): (1) Cluster 1 exhibited up-regulation of GO-BP terms related to ribosome biogenesis and translational control, crucial for maintaining stem cell properties and homeostasis; (2) Additionally, cluster 1 showed up-regulation of GO-BP terms associated with mitochondrial oxidative metabolism; (3) Cluster 3 displayed up-regulation of GO-BP terms related to cell proliferation; (4) Clusters 0 and 2 demonstrated similar up-regulation of GO-BP terms linked to collagen fibril organization and supramolecular fiber organization. However, only cluster 0 showed a significant decrease in GO-BP terms related to ribosome production, implying a potential correlation between ribosome regulation and the differentiation stages of AT-MSCs. Overall, our findings highlight heterogeneous cell clusters with varying balances between proliferation and differentiation before, and after, chondrogenic stimulation. This provides enhanced insights into the single-cell dynamics of AT-MSCs during chondrogenic differentiation. [BMB Reports 2024; 57(5): 232-237]. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | The Biochemical Society of the Republic of Korea | - |
dc.title | Single-cell RNA sequencing reveals the heterogeneity of adipose tissue-derived mesenchymal stem cells under chondrogenic induction | - |
dc.type | Article | - |
dc.identifier.doi | 10.5483/BMBRep.2023-0161 | - |
dc.identifier.bibliographicCitation | BMB reports, v.57, no.5, pp 232 - 237 | - |
dc.identifier.kciid | ART003083461 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85194216205 | - |
dc.citation.endPage | 237 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 232 | - |
dc.citation.title | BMB reports | - |
dc.citation.volume | 57 | - |
dc.type.docType | Note | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Adipose tissue-derived mesenchymal stem cells | - |
dc.subject.keywordAuthor | AT-MSCs | - |
dc.subject.keywordAuthor | Chondrogenesis | - |
dc.subject.keywordAuthor | Heterogeneity | - |
dc.subject.keywordAuthor | Single-cell RNA sequencing | - |
dc.subject.keywordAuthor | scRNAseq | - |
dc.subject.keywordAuthor | Transcriptome | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.