Salinimicrobium soli sp nov., isolated from soil of reclaimed land

Abstract

A Gram-stain-negative, facultatively anaerobic, non-gliding, rod-shaped bacterial strain, designated CAU 1287(T), was isolated from a soil sample of reclaimed land and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1287(T) grew optimally at 30 degrees C, at pH 5.5 and in the presence of up to 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1287(T) formed a distinct lineage within the genus Salinimicrobium and shared the highest 16S rRNA gene sequence similarity of 98.0 % with Salinimicrobium gaetbulicola KCTC 23579(T). Similar to other species of the genus Salinimicrobium, menaquinone-6 (MK-6) was the only isoprenoid quinone and iso-C-15:0 and anteiso-C-15:0 were the major cellular fatty acid detected in strain CAU 1287(T). The polar lipid pattern of strain CAU 1287(T) consisted of phosphatidylethanolamine and various unidentified lipids including three aminolipids, one aminophospholipid, one phosphoglycolipid, one glycolipid, and three lipids. The DNA G+C content of CAU 1287(T) was 43.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CAU 1287(T) represents a novel species of the genus Salinimicrobium, for which the name Salinimicrobium soli sp. nov. is proposed. The type strain is CAU 1287(T) (=KCTC 32163(T)=CCUG 64794(T)).