Efficacy of Nitric Oxide-Releasing Nanofibers in Reducing Renal Ischemia-Reperfusion Injury in a Rat Model
DC Field | Value | Language |
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dc.contributor.author | Ko, Hyunmin | - |
dc.contributor.author | Kim, Jin Sug | - |
dc.contributor.author | Shin, Jae Ho | - |
dc.contributor.author | Jeong, Kyung Hwan | - |
dc.contributor.author | Ahn, Hyung Joon | - |
dc.date.accessioned | 2024-07-08T06:30:31Z | - |
dc.date.available | 2024-07-08T06:30:31Z | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 1425-9524 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74635 | - |
dc.description.abstract | Background: This study aimed to analyze the preventive effect of nitric oxide (NO)-releasing nanofibers against ischemia-reperfusion injury (IRI) and to determine the mechanism of action as a novel NO delivery system in a rat model. Material/Methods: Eight-week-old male Sprague-Dawley rats, weighing 250 to 280 g, were divided into 3 groups: sham, untreated (n=5); control, renal ischemia injury for 55 min (n=4); and NO24, renal ischemia injury for 55 min with kidney wrapping of NO-releasing nanofiber for 24 h (n=6). mRNA expression was measured by real-time polymerase chain reaction (PCR), whereas protein expression was assessed by immunohistochemistry and western blot analysis. Results: Serum creatinine levels in the sham, control, and NO24 groups were 0.48 +/- 0.08, 4.66 +/- 0.33, and 2.60 +/- 1.00 mg/dL, respectively (P=0.002). Anti-apoptotic Bcl-2 protein expression differed significantly between the control and the NO24 groups (Bcl-2/b-actin; control, 0.50 +/- 0.12; NO24, 1.56 +/- 0.56; P=0.024). mRNA expression level of the inflammatory cytokine tumor necrosis factor-a (TNF-a) was significantly higher in the control group (23.24 +/- 11.32, P=0.016) than in the sham group (1.00 +/- 1.21), and mRNA expression of TNF-a in the NO24 group (1.28 +/- 1.16, P=0.010) was significantly lower than in the control group. Histological analysis revealed decreased atrophy and necrosis in the NO24 group compared to those in the control group. Conclusions: This study demonstrated that kidney wrapping of NO-releasing nanofibers had a protective effect against kidney IRI through anti-apoptotic and anti-inflammatory mechanisms. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | INT SCIENTIFIC INFORMATION, INC | - |
dc.title | Efficacy of Nitric Oxide-Releasing Nanofibers in Reducing Renal Ischemia-Reperfusion Injury in a Rat Model | - |
dc.type | Article | - |
dc.identifier.doi | 10.12659/AOT.934800 | - |
dc.identifier.bibliographicCitation | ANNALS OF TRANSPLANTATION, v.27 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000746074900001 | - |
dc.identifier.scopusid | 2-s2.0-85123813460 | - |
dc.citation.title | ANNALS OF TRANSPLANTATION | - |
dc.citation.volume | 27 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Kidney Transplantation | - |
dc.subject.keywordAuthor | Nanofibers | - |
dc.subject.keywordAuthor | Nitric Oxide | - |
dc.subject.keywordPlus | LIVER ISCHEMIA | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | SELECTIN | - |
dc.subject.keywordPlus | INTERLEUKIN-1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CASPASE-3 | - |
dc.subject.keywordPlus | PROTECTS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | KIDNEY | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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