Elucidating Korean meadowsweet (Filipendula glaberrima Nakai)-derived arabinogalactan protein-induced macrophage activation and its associated mechanism of action
DC Field | Value | Language |
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dc.contributor.author | Hong, Mijin | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.contributor.author | Kim, Hoon | - |
dc.contributor.author | Hwang, Dahyun | - |
dc.date.accessioned | 2024-07-12T05:30:38Z | - |
dc.date.available | 2024-07-12T05:30:38Z | - |
dc.date.issued | 2024-07 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.issn | 1879-0003 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74717 | - |
dc.description.abstract | This study aimed to confirm macrophage-stimulatory component from Korean meadowsweet (Filipendula glaberrima; FG) and characterize its compositional and structural properties. FG-CWH, prepared via cool-water extraction and ethanol precipitation, induced the highest secretion of NO (6.0–8.0 μM), TNF-α (8.7–9.5 ng/mL), and IL-6 (1.0–5.7 ng/mL) compared to other samples at 0.4–10 μg/mL in RAW 264.7 cells. Analytical results revealed that FG-CWH is a high-molecular-weight component with an average molecular weight of 220 kDa, constituting a polysaccharide-protein mixture. Chemical and enzymatic treatment of FG-CWH indicated its primary composition as arabinogalactan protein (AGP)-rich glycoprotein, with activity likely associated with the chemical and structural characteristics of AGP. FG-CWH treatment resulted in significant and concentration-dependent increases in iNOS (20.0–29.6 folds), TNFα (10.6–18.6 folds) and IL6 (10.9–155.6 folds) gene expression, as well as the secretion of NO (5.3–6.3 μM), TNF-α (35.4–44.3 ng/mL), and IL-6 (4.1–8.4 ng/mL) secretion, even at a reduced concentration range of 125–500 ng/mL, compared to the negative control group. Immunoblotting analysis indicated FG-CWH-induced macrophage stimulation significantly associated with the activation of MAPK (ERK, JNK, and p38) and NF-κB (p65 and IκBα). These findings can serve as valuable groundwork for developing FG-derived AGP as novel functional ingredients to enhance human immunity. © 2024 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Elucidating Korean meadowsweet (Filipendula glaberrima Nakai)-derived arabinogalactan protein-induced macrophage activation and its associated mechanism of action | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2024.132999 | - |
dc.identifier.bibliographicCitation | International Journal of Biological Macromolecules, v.273 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 001257780900001 | - |
dc.identifier.scopusid | 2-s2.0-85195668855 | - |
dc.citation.title | International Journal of Biological Macromolecules | - |
dc.citation.volume | 273 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Glycoprotein | - |
dc.subject.keywordAuthor | Immunostimulation | - |
dc.subject.keywordAuthor | Macrophage | - |
dc.subject.keywordAuthor | MAPK/NF-κB | - |
dc.subject.keywordAuthor | Polysaccharide | - |
dc.subject.keywordAuthor | RAW 264.7 | - |
dc.subject.keywordPlus | SYSTEM MODULATING POLYSACCHARIDES | - |
dc.subject.keywordPlus | ULMARIA L. MAXIM. | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | FILIPENDULA-VULGARIS | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | INNATE | - |
dc.subject.keywordPlus | ACIDS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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