Comprehensive Study of Microsatellite Instability Testing and Its Comparison With Immunohistochemistry in Gastric Cancers
DC Field | Value | Language |
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dc.contributor.author | Park, Yujun | - |
dc.contributor.author | Nam, Soo Kyung | - |
dc.contributor.author | Seo, Soo Hyun | - |
dc.contributor.author | Park, Kyoung Un | - |
dc.contributor.author | Oh, Hyeon Jeong | - |
dc.contributor.author | Park, Young Suk | - |
dc.contributor.author | Suh, Yun-Suhk | - |
dc.contributor.author | Ahn, Sang-Hoon | - |
dc.contributor.author | Park, Do Joong | - |
dc.contributor.author | Kim, Hyung-Ho | - |
dc.contributor.author | Lee, Hye Seung | - |
dc.date.accessioned | 2024-07-17T03:00:29Z | - |
dc.date.available | 2024-07-17T03:00:29Z | - |
dc.date.issued | 2023-04 | - |
dc.identifier.issn | 2093-582X | - |
dc.identifier.issn | 2093-5641 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74853 | - |
dc.description.abstract | Purpose: In this study, polymerase chain reaction (PCR)-based microsatellite instability (MSI) testing was comprehensively analyzed and compared with immunohistochemistry (IHC) for mismatch repair (MMR) protein expression in patients with gastric cancer (GC). Materials and Methods: In 5,676 GC cases, PCR-based MSI testing using five microsatellites (BAT-26, BAT-25, D5S346, D2S123, and D17S250) and IHC for MLH1 were performed. Re-evaluation of MSI testing/MLH1 IHC and additional IHC for MSH2, MSH6, and PMS2 were performed in discordant/indeterminate cases.Results: Of the 5,676 cases, microsatellite stable (MSS)/MSI-low and intact MLH1 were observed in 5,082 cases (89.5%), whereas MSI-high (MSI-H) and loss of MLH1 expression were observed in 502 cases (8.8%). We re-evaluated the remaining 92 cases (1.6%) with a discordant/ indeterminate status. Re-evaluation showed 1) 37 concordant cases (0.7%) (18 and 19 cases of MSI-H/MMR-deficient (dMMR) and MSS/MMR-proficient (pMMR), respectively), 2) 6 discordant cases (0.1%) (3 cases each of MSI-H/pMMR and MSS/dMMR), 3) 14 MSI indeterminate cases (0.2%) (1 case of dMMR and 13 cases of pMMR), and 4) 35 IHC indeterminate cases (0.6%) (22 and 13 cases of MSI-H and MSS, respectively). Finally, MSI-H or dMMR was observed in 549 cases (9.7%), of which 47 (0.8%) were additionally confirmed as MSI-H or dMMR by re-evaluation. Sensitivity was 99.3% for MSI testing and 95.4% for MMR IHC.Conclusions: Considering the low incidence of MSI-H or dMMR, discordant/indeterminate results were occasionally identified in GCs, in which case complementary testing is required. These findings could help improve the accuracy of MSI/MMR testing in daily practice. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN GASTRIC CANCER ASSOC | - |
dc.title | Comprehensive Study of Microsatellite Instability Testing and Its Comparison With Immunohistochemistry in Gastric Cancers | - |
dc.type | Article | - |
dc.identifier.doi | 10.5230/jgc.2023.23.e5 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GASTRIC CANCER, v.23, no.2, pp 264 - 274 | - |
dc.identifier.kciid | ART002956980 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000997962300003 | - |
dc.identifier.scopusid | 2-s2.0-85160846096 | - |
dc.citation.endPage | 274 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 264 | - |
dc.citation.title | JOURNAL OF GASTRIC CANCER | - |
dc.citation.volume | 23 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Microsatellite instability | - |
dc.subject.keywordAuthor | DNA mismatch repair | - |
dc.subject.keywordAuthor | Immunohistochemistry | - |
dc.subject.keywordAuthor | Gastric cancer | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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