Genetic and immune microenvironment characterization of HER2-positive gastric cancer: Their association with response to trastuzumab-based treatmentopen access
- Authors
- Kwon, Hyun Jung; Park, Yujun; Nam, Soo Kyung; Kang, Enoch; Kim, Ka-Kyung; Jeong, Inhae; Kwak, Yoonjin; Yoon, Jeesun; Kim, Tae-Yong; Lee, Keun-Wook; Oh, Do-Youn; Im, Seock-Ah; Kong, Seong-Ho; Park, Do Joong; Lee, Hyuk-Joon; Kim, Hyung-Ho; Yang, Han-Kwang; Lee, Hye Seung
- Issue Date
- May-2023
- Publisher
- WILEY
- Keywords
- cell cycle; HER2-positive gastric cancer; NK cell; PD-L1; trastuzumab
- Citation
- CANCER MEDICINE, v.12, no.9, pp 10371 - 10384
- Pages
- 14
- Journal Title
- CANCER MEDICINE
- Volume
- 12
- Number
- 9
- Start Page
- 10371
- End Page
- 10384
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/74988
- DOI
- 10.1002/cam4.5769
- ISSN
- 2045-7634
- Abstract
- Background: We aimed to determine the molecular and immune microenvironment characteristics of HER2-positive gastric cancer (GC) related to the patient's response to first line trastuzumab- based treatment.Methods: Eighty three cases of HER2-positive advanced gastric adenocarcinoma patients treated with trastuzumab were enrolled. Targeted deep sequencing and transcriptome analysis were performed on selected 21 cases (exploration cohort) along with two post-treatment samples. The results were compared between patients progressed before 6 months (Group 2) and others (Group 1), and were validated by FISH and immunohistochemistry in total cohort. Tumor-infiltrating immune cells were evaluated using RNA sequencing data and multiplex immunohistochemistry. Progression free survival (PFS) analysis was performed.Results: Group 1 showed frequent amplification of G1/S cell cycle checkpoint related genes and upregulated KEGG pathways related to cell proliferation. In contrast, Group 2 had more frequent EGFR, HER3, and MET amplification and higher RNA expression in immune-related KEGG pathways than Group 1. In total cohort, significant predictors of better PFS were cell cycle-related including CCNE1 amplification, Cyclin A and PLK1 overexpression, and decreased Cyclin D3 and HER3 expression (p < 0.05), or immune-related including high density of CD3(-)CD57(+) NK cells and PD -L1 combined positive score >= 5 (p < 0.05). The best prognostic predictors were a combination of Cyclin A, Cyclin E, p21, and HER3 (p < 0.001).Conclusion: HER2-positive GC with favorable response to trastuzumab were characterized by cell cycle-related gene alterations and increased CD3-CD57+ NK cell infiltration. These findings would be helpful to the fine modulation of therapeutic strategies for patients with HER2-positive GC.
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