Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Russell, Jordan | - |
dc.contributor.author | Kim, Sun-Ki | - |
dc.contributor.author | Duma, Justin | - |
dc.contributor.author | Nothaft, Harald | - |
dc.contributor.author | Himmel, Michael E. | - |
dc.contributor.author | Bomble, Yannick J. | - |
dc.contributor.author | Szymanski, Christine M. | - |
dc.contributor.author | Westpheling, Janet | - |
dc.date.available | 2019-01-22T12:38:08Z | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/787 | - |
dc.description.abstract | Protein glycosylation pathways have been identified in a variety of bacteria and are best understood in pathogens and commensals in which the glycosylation targets are cell surface proteins, such as S layers, pili, and flagella. In contrast, very little is known about the glycosylation of bacterial enzymes, especially those secreted by cellulolytic bacteria. Caldicellulosiruptor bescii secretes several unique synergistic multifunctional biomass-degrading enzymes, notably cellulase A which is largely responsible for this organism's ability to grow on lignocellulosic biomass without the conventional pretreatment. It was recently discovered that extracellular CelA is heavily glycosylated. In this work, we identified an O-glycosyltransferase in the C. bescii chromosome and targeted it for deletion. The resulting mutant was unable to grow on crystalline cellulose and showed no detectable protein glycosylation. Multifunctional biomass-degrading enzymes in this strain were rapidly degraded. With the genetic tools available in C. bescii, this system represents a unique opportunity to study the role of bacterial enzyme glycosylation as well an investigation of the pathway for protein glycosylation in a non-pathogen. | - |
dc.publisher | BMC | - |
dc.title | Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s13068-018-1266-x | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY FOR BIOFUELS, v.11, no.1 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000445749400001 | - |
dc.identifier.scopusid | 2-s2.0-85053873131 | - |
dc.citation.number | 1 | - |
dc.citation.title | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.volume | 11 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | CAMPYLOBACTER-JEJUNI | - |
dc.subject.keywordPlus | PLANT BIOMASS | - |
dc.subject.keywordPlus | DSM 6725 | - |
dc.subject.keywordPlus | ANAEROCELLUM-THERMOPHILUM | - |
dc.subject.keywordPlus | N-GLYCOSYLATION | - |
dc.subject.keywordPlus | O-MANNOSYLATION | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | DOMAINS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.