A Simple Pipetting-based Method for Encapsulating Live Cells into Multi-layered Hydrogel Droplets
- Authors
- Yeon, Ju Hun; Chung, Sung Hee; Baek, Changyoon; Hwang, Hyundoo; Min, Junhong
- Issue Date
- Sep-2018
- Publisher
- KOREAN BIOCHIP SOCIETY-KBCS
- Keywords
- Multi-layered; Hydrogel; Droplets; Encapsulate; HEK293 cell; Drug assays
- Citation
- BIOCHIP JOURNAL, v.12, no.3, pp 184 - 192
- Pages
- 9
- Journal Title
- BIOCHIP JOURNAL
- Volume
- 12
- Number
- 3
- Start Page
- 184
- End Page
- 192
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/842
- DOI
- 10.1007/s13206-018-2307-z
- ISSN
- 1976-0280
2092-7843
- Abstract
- Here, we present a simple pipetting-based approach for generating multi-layered core-shell hydrogel droplets composed of alginate for outer shell and collagen for culturing cells inside. By using a multi-hole plastic substrate and by pipetting, multi-layered hydrogel droplets were generated in a simple and rapid manner. HEK293 cells, which are human embryonic kidney cells, were cultured for 14 days in double-layered hydrogel droplet with high viability. Cancer cells were co-cultured with epithelial cells in multi-layered hydrogel droplets and applied for drug tests with curcumin. As epithelial cells protect cancer cells from anti-cancer drugs, co-cultured cells showed lower sensitivity to curcumin. We developed a simple and easy method for creating complex hydrogel particles for 3D multicellular co-culture and developed an alternative method for drug testing in vivo.
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Collections - College of ICT Engineering > School of Integrative Engineering > 1. Journal Articles
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