잔대 추출물과 이들 분획물들의 항산화 및 뇌신경세포 보호 효과
DC Field | Value | Language |
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dc.contributor.author | Chung, M.J. | - |
dc.contributor.author | Lee, S. | - |
dc.contributor.author | Park, Y.I. | - |
dc.contributor.author | Kwon, K.H. | - |
dc.date.available | 2019-03-08T15:40:33Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1226-3311 | - |
dc.identifier.issn | 2288-5978 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/8577 | - |
dc.description.abstract | 잔대의 70% 에탄올 추출물은 강한 항산화 효과 및 산화적 스트레스 조건에서 뇌신경세포 사멸 억제 효과가 있었다. 6개의 분획물 중에 잔대 추출물의 클로로포름 분획물(ATCH)은 높은 DPPH 라디칼 소거작용 및 세포 내 활성산소종(ROS) 소거작용을 가지고 있었고 H₂O₂에 대항하여 뇌신경세포(SK-N-SH) 보호 효과가 가장 뛰어났다. 활성이 가장 우수한 클로로포름 분획물을 대상으로 주요 물질을 순수분리한 후 ¹H-NMR, 13C-NMR 그리고 EI mass spectra를 이용하여 화학구조를 분석한 결과 phytosterols(β-sitosterol과 daucosterol)이라는 것을 알 수 있었다. β-Sitosterol과 daucosterol 역시 산화적 스트레스에 대항하여 SK-NSH세포를 보호하는 작용이 있었다. AT-CH 그리고 이것으로부터 분리된 화합물은 p38 경로를 저해하였다. 이들 결과는 AT-CH는 p38 경로를 저해하고 세포 내 ROS 소거작용에 의해 산화적 스트레스로부터 뇌신경세포 보호 효과를 가질 것이라 제안하였다. | - |
dc.description.abstract | The 70% ethanol extract from Adenophora triphylla showed a strong antioxidant effect and reduced cytotoxicity of H2O2 in SK-N-SH cells. The chloroform fraction from A. triphylla extract (AT-CH) among the six fractions showed strong DPPH radical and intracellular reactive oxygen species (ROS) scavenger effects and the highest protective effect against H2O2-induced SK-N-SH cell death. Bioactivity compounds were purified from AT-CH, and the chemical structures of the compounds were determined as β-sitosterol and daucosterol on the basis of 1H-NMR, 13C-NMR, and EI mass spectra. β-Sitosterol and daucosterol also had protective effects against oxidative stress in SK-N-SH cells. Phospho-p38 MAPK levels were elevated by H2O2 but were inhibited by treatment with AT-CH and phytosterols (β-sitosterol and daucosterol) isolated from AT-CH. These results suggest that AT-CH has brain neuroprotective effects against oxidative stress (H2O2) by inhibiting activation of p38 pathways and scavenging intracellular ROS. | - |
dc.format.extent | 9 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | Korean Society of Food Science and Nutrition | - |
dc.title | 잔대 추출물과 이들 분획물들의 항산화 및 뇌신경세포 보호 효과 | - |
dc.title.alternative | Antioxidative and neuroprotective effects of extract and fractions from Adenophora triphylla | - |
dc.type | Article | - |
dc.identifier.doi | 10.3746/jkfn.2016.45.11.1580 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society of Food Science and Nutrition, v.45, no.11, pp 1580 - 1588 | - |
dc.identifier.kciid | ART002168547 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85007233407 | - |
dc.citation.endPage | 1588 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1580 | - |
dc.citation.title | Journal of the Korean Society of Food Science and Nutrition | - |
dc.citation.volume | 45 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Adenophora triphylla | - |
dc.subject.keywordAuthor | Antioxidant effect | - |
dc.subject.keywordAuthor | Neuroprotective effects | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | SK-N-SH cells | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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