Design of Multifunctional Liposomal Nanocarriers for Folate Receptor-Specific Intracellular Drug Delivery
DC Field | Value | Language |
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dc.contributor.author | Kang, Min Hyung | - |
dc.contributor.author | Yoo, Hyun Joon | - |
dc.contributor.author | Kwon, Yie Hyuk | - |
dc.contributor.author | Yoon, Ho Yub | - |
dc.contributor.author | Lee, Sang Gon | - |
dc.contributor.author | Kim, Sung Rae | - |
dc.contributor.author | Yeom, Dong Woo | - |
dc.contributor.author | Kang, Myung Joo | - |
dc.contributor.author | Choi, Young Wook | - |
dc.date.available | 2019-03-08T16:00:56Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 1543-8384 | - |
dc.identifier.issn | 1543-8392 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/8860 | - |
dc.description.abstract | As a novel carrier for folate receptor (FR)-targeted intracellular delivery, we designed two types of targetable liposomal systems using Pep-1 peptide (Pep1) and folic acid as a cell-penetrating peptide (CPP) and target molecule, respectively. Folate-linked Pep1 (Fol-Pep1) was synthesized by solid phase peptide synthesis (SPPS) and verified using H-1 NMR and farultraviolet (UV) circular dichroism (CD). The chimeric ligand (Fol-Pep1)-modified liposome (cF-P-L) was prepared by coupling Fol-Pep1 to maleimide-derivatized liposomes at various ratios. The dual ligand (folate and Pep 1)-modified liposome (dF/P-L) was prepared by separately attaching both ligands to the liposomal surface via a short (PEG(2000)) or long (PEG(3400)) linker. The physical and conformational characteristics including vesicle size, zeta potential, and the number of conjugated ligands were determined. Intracellular uptake specificities of various fluorescent probe-containing cF-P-L and dF/P-L systems were assessed using FR-positive HeLa and FR-negative HaCaT cells. Cellular uptake behavior was visualized by confocal laser scanning microscopy (CLSM). Internalization was time-dependent. Fol-Pep1 and Pep-1 cytotoxicities were negligible up to 25 mu M in FR-positive and FR-negative cells. Empty cF-P-L and dF/P-L were nontoxic at the concentration used. The optimized dF(3)/P-2(450/90) system carrying 450 PEG(3400)-linked folate and 90 PEG(2000)-linked Pep1 molecules could be a good candidate for FR-specific intracellular drug delivery. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Design of Multifunctional Liposomal Nanocarriers for Folate Receptor-Specific Intracellular Drug Delivery | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.molpharmaceut.5b00399 | - |
dc.identifier.bibliographicCitation | MOLECULAR PHARMACEUTICS, v.12, no.12, pp 4200 - 4213 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000366151500003 | - |
dc.identifier.scopusid | 2-s2.0-84949024561 | - |
dc.citation.endPage | 4213 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 4200 | - |
dc.citation.title | MOLECULAR PHARMACEUTICS | - |
dc.citation.volume | 12 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Liposome | - |
dc.subject.keywordAuthor | cell penetrating peptide | - |
dc.subject.keywordAuthor | drug targeting Pep-1 | - |
dc.subject.keywordAuthor | dual-ligand | - |
dc.subject.keywordAuthor | folic acid | - |
dc.subject.keywordAuthor | intracellular delivery | - |
dc.subject.keywordPlus | CELL-PENETRATING PEPTIDES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | SECONDARY STRUCTURE | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | FOLIC-ACID | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CARRIER | - |
dc.subject.keywordPlus | PEP-1 | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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