Robust fluorescence sensing platform for detection of CD44 cells based on graphene oxide/gold nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Jeong, Ha Young | - |
dc.contributor.author | Baek, Seung Hun | - |
dc.contributor.author | Chang, Sung-Jin | - |
dc.contributor.author | Cheon, Seon Ah | - |
dc.contributor.author | Park, Tae Jung | - |
dc.date.available | 2019-03-08T16:37:00Z | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.issn | 1873-4367 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/8925 | - |
dc.description.abstract | Gold-coated graphene oxide hybrid material (GO/AuNPs) has exceptional physical and chemical properties like pi-pi stacking interaction and plays a role in quencher of fluorescence dye. Therefore, GO/AuNPs could enhance the signal-to-background ratio with fluorescence dye that was the point in this fluorescent biosensor. In this study, tetramethy1-6-carboxy-rhodamine (TAMRA)-labeled aptamers that specifically interact with the hyaluronic acid binding domain of CD44 were used as targets to investigate the applicability of the method. GO/AuNPs TAMRA-aptamer complexes could detect CD44 target cancer cells within a concentration range of 1 x 10(1) to 1 x 10(7) CFU/mL. A linear relationship was observed between target cell concentration and relative fluorescence intensity. The more mounted up CD44 target cell concentrations, relative fluorescence intensity of GO/AuNPs TAMRA-aptamer complexes was increased even more, which was superior to that of GO alone. Sensitivity of the detection system displayed a low detection limit of 1 x 10(1) CFU/mL. Additionally, this method is specific in that fluorescence is not much enhanced in CD44 negative cancer cell line. Thus, the fluorescence sensing based on GO/AuNPs could be developed to receptive and robust detection tool for various target molecules. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Robust fluorescence sensing platform for detection of CD44 cells based on graphene oxide/gold nanoparticles | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.colsurfb.2015.07.083 | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.135, pp 309 - 315 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000367410200036 | - |
dc.identifier.scopusid | 2-s2.0-84938778226 | - |
dc.citation.endPage | 315 | - |
dc.citation.startPage | 309 | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 135 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Graphene oxide/gold nanoparticles (GO/AuNPs) | - |
dc.subject.keywordAuthor | Fluorescence sensing | - |
dc.subject.keywordAuthor | Signal-to-background ratio enhancer | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Hyaluronic acid binding domain of CD44 | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | SIGNAL AMPLIFICATION | - |
dc.subject.keywordPlus | EXONUCLEASE III | - |
dc.subject.keywordPlus | DNA ANALYSIS | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | CONDUCTIVITY | - |
dc.subject.keywordPlus | NANOPROBE | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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