Effects of selective cleavage of high-mannose-type glycans of Maackia amurensis leukoagglutinin on sialic acid-binding activity
DC Field | Value | Language |
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dc.contributor.author | Kim, Byung Sun | - |
dc.contributor.author | Hwang, Hye Seong | - |
dc.contributor.author | Park, Heajin | - |
dc.contributor.author | Kim, Ha Hyung | - |
dc.date.available | 2019-03-08T16:41:06Z | - |
dc.date.issued | 2015-09 | - |
dc.identifier.issn | 0304-4165 | - |
dc.identifier.issn | 1872-8006 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9143 | - |
dc.description.abstract | Background: Maackia amurensis leukoagglutinin (MAL) is a glycoprotein and sialic arid-binding lectin that is used widely in the detection and characterization of sialoglycoconjugates and human cancer cells. However, its N-linked glycan structure and role have yet to be determined. Methods: The N-linked glycans were analyzed using high-performance liquid chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and the secondary structure was investigated using circular dichroism analysis. A hemagglutination assay was performed. Furthermore, surface plasmon resonance analysis, and fluorescence microscopy and fluorescence-activated cell-sorting analysis were conducted to assess the sialoglycoprotein-binding ability and its usefulness in the detection of human breast cancer MCF-7 cells, respectively. Results: Analysis of the N-linked glycan structure of MAL confirmed the presence of eight glycans, comprising two alpha 1,3-fucosylated paucimannosidic-type and six high-mannose-type glycans. Glycan analysis of MAL that had been treated with peptide N-glycosidase F (de-M-MAL) revealed that while the two alpha 1,3-fucosylated paucimannosidic glycans remained attached following the treatment, the six high-mannose-type glycans had been completely cleaved from the original MAL There were almost no secondary structural changes between MAL and de-M-MAL; however, the lectin activities exhibited by MAL, such as hemagglutination and binding to a sialoglycoprotein, were completely absent in de-M-MAL, and the ability to detect human breast cancer MCF-7 cells was 77% lower in de-M-MAL than in MAL. Conclusion: The high-mannose-type glycans in intact MAL are closely associated with its lectin activities. General significance: This is the first report of the N-linked glycan structure of MAL and the effect of high-mannose-type glycans on lectin activities. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Effects of selective cleavage of high-mannose-type glycans of Maackia amurensis leukoagglutinin on sialic acid-binding activity | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbagen.2015.05.011 | - |
dc.identifier.bibliographicCitation | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v.1850, no.9, pp 1815 - 1821 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000359173900017 | - |
dc.identifier.scopusid | 2-s2.0-84930615876 | - |
dc.citation.endPage | 1821 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1815 | - |
dc.citation.title | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | - |
dc.citation.volume | 1850 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Maackia amurensis lectin | - |
dc.subject.keywordAuthor | N-linked glycan | - |
dc.subject.keywordAuthor | High-mannose-type | - |
dc.subject.keywordAuthor | Activity | - |
dc.subject.keywordPlus | N-GLYCANS | - |
dc.subject.keywordPlus | CHEMICAL-MODIFICATION | - |
dc.subject.keywordPlus | H-1-NMR SPECTROSCOPY | - |
dc.subject.keywordPlus | STRUCTURAL-ANALYSIS | - |
dc.subject.keywordPlus | CARBOHYDRATE CHAIN | - |
dc.subject.keywordPlus | LECTINS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | RESIDUES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACETYLGLUCOSAMINE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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