Low concentrations of doxycycline attenuates FasL-induced apoptosis in HeLa cells
DC Field | Value | Language |
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dc.contributor.author | Yoon, Jung Mi | - |
dc.contributor.author | Koppula, Sushruta | - |
dc.contributor.author | Huh, Se Jong | - |
dc.contributor.author | Hur, Sun Jin | - |
dc.contributor.author | Kim, Chan Gil | - |
dc.date.available | 2019-03-08T16:59:00Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 0716-9760 | - |
dc.identifier.issn | 0717-6287 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9334 | - |
dc.description.abstract | Background: Doxycycline (DC) has been shown to possess non-antibiotic properties including Fas/Fas Ligand (FasL)-mediated apoptosis against several tumor types in the concentration range of 10-40 mu g/mL. However, the effect of DC in apoptotic signaling at much low concentrations was not studied. Methods: The present study investigated the attenuation effect of low dose of DC on FasL-induced apoptosis in HeLa cell by the methods of MTT assay, fluorescence microscopy, DNA fragmentation, flow cytometry analysis, and western blotting. Results and conclusion: In the present findings we showed that low concentration of DC (<2.0 mu g/mL) exhibited protective effects against FasL-induced apoptosis in HeLa cells. FasL treatment to HeLa cells resulted in a concentration-dependent induction of cell death, and treatment with low concentrations of DC (0.1-2 mu g/mL) significantly (p < 0.001) attenuated the FasL-induced cell death as measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the FasL-induced apoptotic features in HeLa cells, such as morphological changes, DNA fragmentation and cell cycle arrest was also inhibited by DC (0.5 mu g/mL). Tetracycline and minocycline also showed similar anti-apoptotic effects but were not significant when compared to DC, tested at same concentrations. Further, DC (0.01-16 mu g/mL) did not influence the hydrogen peroxide-or cisplatin-induced intrinsic apoptotic pathway in HeLa cells. Protein analysis using Western blotting confirmed that FasL-induced cleavage/activation of caspase-8 and caspase-3, were inhibited by DC treatment at low concentration (0.5 mu g/mL). Considering the overall data, we report for the first time that DC exhibited anti-apoptotic effects at low concentrations in HeLa cells by inhibition of caspase activation via FasL-induced extrinsic pathway. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SOC BIOLGIA CHILE | - |
dc.title | Low concentrations of doxycycline attenuates FasL-induced apoptosis in HeLa cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s40659-015-0025-8 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL RESEARCH, v.48 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000358475600001 | - |
dc.identifier.scopusid | 2-s2.0-84938775726 | - |
dc.citation.title | BIOLOGICAL RESEARCH | - |
dc.citation.volume | 48 | - |
dc.type.docType | Article | - |
dc.publisher.location | 칠레 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Caspase | - |
dc.subject.keywordAuthor | Cisplatin | - |
dc.subject.keywordAuthor | FasL | - |
dc.subject.keywordAuthor | Hydrogen peroxide | - |
dc.subject.keywordAuthor | Tetracycline | - |
dc.subject.keywordPlus | PANCREATIC-CANCER CELLS | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | TETRACYCLINE | - |
dc.subject.keywordPlus | MINOCYCLINE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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