Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Donghoon | - |
dc.contributor.author | Lee, Sanghee | - |
dc.contributor.author | Ahn, Myung Mo | - |
dc.contributor.author | Kang, In Seok | - |
dc.contributor.author | Park, Ki-Hwan | - |
dc.contributor.author | Jeon, Sangmin | - |
dc.date.available | 2019-03-08T16:59:15Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.issn | 1873-4324 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9349 | - |
dc.description.abstract | A colorimetric method that uses platinum-coated magnetic nanoparticle clusters (Pt/MNCs) and magnetophoretic chromatography is developed to detect pathogenic bacteria. Half-fragments of monoclonal Escherichia coli O157:H7 (EC) antibodies were functionalized to Pt/MNCs and used to capture Escherichia coli bacteria in milk. After magnetic separation of free Pt/MNCs and Pt/MNC-EC complexes from the milk, a precision pipette was used to imbibe the Escherichia coli-containing solution, then a viscous polyethylene glycol solution. Due to difference in viscosities, the solutions separate into two liquid layers inside the pipette tip. The Pt/MNC-EC complexes were separated from the free Pt/MNCs by applying an external magnetic field, then added to a tetramethylbenzidine (TMB) solution. Catalytic oxidation of TMB by Pt produced color changes of the solution, which enabled identification of the presence of 10 cfu mL(-1) Escherichia coli bacteria with the naked eye. The total assay time including separation, binding and detection was 30 min. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.aca.2015.04.044 | - |
dc.identifier.bibliographicCitation | ANALYTICA CHIMICA ACTA, v.883, pp 61 - 66 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000356236700007 | - |
dc.identifier.scopusid | 2-s2.0-84931006192 | - |
dc.citation.endPage | 66 | - |
dc.citation.startPage | 61 | - |
dc.citation.title | ANALYTICA CHIMICA ACTA | - |
dc.citation.volume | 883 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Magnetophoretic chromatography | - |
dc.subject.keywordAuthor | Pathogenic bacteria | - |
dc.subject.keywordAuthor | Platinum nanoparticles | - |
dc.subject.keywordAuthor | Colorimetric detection | - |
dc.subject.keywordPlus | SALMONELLA-TYPHIMURIUM | - |
dc.subject.keywordPlus | LISTERIA-MONOCYTOGENES | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | MILK | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.