Deglycosylation of isoflavone C-glycosides by newly isolated human intestinal bacteria
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Mihyang | - |
dc.contributor.author | Lee, Jaekwan | - |
dc.contributor.author | Han, Jaehong | - |
dc.date.available | 2019-03-08T16:59:37Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 0022-5142 | - |
dc.identifier.issn | 1097-0010 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9370 | - |
dc.description.abstract | BACKGROUNDPlant isoflavones are mostly present in the glycoside form. Isoflavone aglycones produced by intestinal microflora are reported to be more bioactive than the glycoside form. However, the deglycosylation of isoflavone C-glycosides is known to be rare, and is less studied. RESULTSThree new bacteria were isolated from human faecal samples, two of which hydrolysed the C-glycosidic bond of puerarin, daidzein-8-C-glucoside. They were identified as two Lactococcus species, herein designated as MRG-IFC-1 and MRG-IFC-3, and an Enterococcus species, herein designated MRG-IFC-2, based on their 16S rDNA sequences. From a reactivity study, it was found that Lactococcus sp. MRG-IFC-1 and Enterococcus sp. MRG-IFC-2 hydrolysed isoflavone C- and O-glycosides, as well as the flavone O-glycoside apigetrin, but could not hydrolyse the flavone C-glycosidic bond of vitexin. The other Lactococcus sp., MRG-IF-3, could not hydrolyse the C-glycosidic linkage of puerarin, while it showed a broad substrate spectrum of O-glycosidase activity similar to the other two bacteria. Puerarin was completely converted to daidzein within 100min by Lactococcus sp. MRG-IFC-1 and Enterococcus sp. MRG-IFC-2, which is the fastest conversion among the reported human intestinal bacteria. CONCLUSIONTwo new puerarin-metabolising human intestinal bacteria were isolated and identified, and the deglycosylation activity for various flavonoid glycosides was investigated. The results could facilitate the study of C-glycosidase reaction mechanisms, as well as the pharmacokinetics of bioactive C-glycoside natural products. (c) 2014 Society of Chemical Industry | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Deglycosylation of isoflavone C-glycosides by newly isolated human intestinal bacteria | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jsfa.6900 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, v.95, no.9, pp 1925 - 1931 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000355630400020 | - |
dc.identifier.scopusid | 2-s2.0-84930677473 | - |
dc.citation.endPage | 1931 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1925 | - |
dc.citation.title | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | - |
dc.citation.volume | 95 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | deglycosylation | - |
dc.subject.keywordAuthor | human intestinal bacteria | - |
dc.subject.keywordAuthor | isoflavone C-glycoside | - |
dc.subject.keywordAuthor | isoflavone O-glycoside | - |
dc.subject.keywordAuthor | puerarin | - |
dc.subject.keywordPlus | ABSOLUTE-CONFIGURATION | - |
dc.subject.keywordPlus | SOY ISOFLAVONES | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | PUERARIN | - |
dc.subject.keywordPlus | (3S)-EQUOL | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | BIOTRANSFORMATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | GLUCOSIDES | - |
dc.subject.keywordPlus | MICROFLORA | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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