H3K9 methyltransferase G9a negatively regulates UHRF1 transcription during leukemia cell differentiation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Kee-Beom | - |
dc.contributor.author | Son, Hye-Ju | - |
dc.contributor.author | Choi, Sulji | - |
dc.contributor.author | Hahm, Ja Young | - |
dc.contributor.author | Jung, Hyeonsoo | - |
dc.contributor.author | Baek, Hee Jo | - |
dc.contributor.author | Kook, Hoon | - |
dc.contributor.author | Hahn, Yoonsoo | - |
dc.contributor.author | Kook, Hyun | - |
dc.contributor.author | Seo, Sang-Beom | - |
dc.date.available | 2019-03-08T17:39:21Z | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.issn | 1362-4962 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9647 | - |
dc.description.abstract | Histone H3K9 methyltransferase (HMTase) G9a-mediated transcriptional repression is a major epigenetic silencing mechanism. UHRF1 (ubiquitin-like with PHD and ring finger domains 1) binds to hemimethylated DNA and plays an essential role in the maintenance of DNA methylation. Here, we provide evidence that UHRF1 is transcriptionally downregulated by H3K9 HMTase G9a. We found that increased expression of G9a along with transcription factor YY1 specifically represses UHRF1 transcription during TPA-mediated leukemia cell differentiation. Using ChIP analysis, we found that UHRF1 was among the transcriptionally silenced genes during leukemia cell differentiation. Using a DNA methylation profiling array, we discovered that the UHRF1 promoter was hypomethylated in samples from leukemia patients, further supporting its overexpression and oncogenic activity. Finally, we showed that G9a regulates UHRF1-mediated H3K23 ubiquitination and proper DNA replication maintenance. Therefore, we propose that H3K9 HMTase G9a is a specific epigenetic regulator of UHRF1. | - |
dc.format.extent | 15 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | H3K9 methyltransferase G9a negatively regulates UHRF1 transcription during leukemia cell differentiation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/nar/gkv183 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.43, no.7, pp 3509 - 3523 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000354722500017 | - |
dc.identifier.scopusid | 2-s2.0-84930507863 | - |
dc.citation.endPage | 3523 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 3509 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 43 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | HEMI-METHYLATED DNA | - |
dc.subject.keywordPlus | SRA PROTEIN UHRF1 | - |
dc.subject.keywordPlus | HISTONE H3 | - |
dc.subject.keywordPlus | CANCER PROGRESSION | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | GLP | - |
dc.subject.keywordPlus | UBIQUITINATION | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | ACETYLATION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.