Induction of Apoptosis by Citrus unshiu Peel in Human Breast Cancer MCF-7 Cells: Involvement of ROS-Dependent Activation of AMPK
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Min Yeong | - |
dc.contributor.author | Bo, Hyun Hwang | - |
dc.contributor.author | Choi, Eun Ok | - |
dc.contributor.author | Kwon, Da He | - |
dc.contributor.author | Kim, Hong Jae | - |
dc.contributor.author | Ahn, Kyu Im | - |
dc.contributor.author | Ji, Seon Yeong | - |
dc.contributor.author | Jeong, Jin-Woo | - |
dc.contributor.author | Park, Shin-Hyung | - |
dc.contributor.author | Hong, Su-Hyun | - |
dc.contributor.author | Kim, Gi-Young | - |
dc.contributor.author | Park, Cheol | - |
dc.contributor.author | Kim, Heui-Soo | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.contributor.author | Yun, Seok-Joong | - |
dc.contributor.author | Kim, Wun Jae | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.date.available | 2019-01-22T13:29:22Z | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.issn | 1347-5215 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/968 | - |
dc.description.abstract | The fruit of Citrus unshiu MARKOVICH used for various purposes in traditional medicine has various pharmacological properties including antioxidant, anti-inflammatory, and antibacterial effects. Recently, the possibility of anti-cancer activity of the extracts or components of this fruit has been reported; however, the exact mechanism has not yet been fully understood. In this study, we evaluated the anti-proliferative effect of water extract of C. unshiu peel (WECU) on human breast cancer MCF-7 cells and investigated the underlying mechanism. Our results showed that reduction of MCF-7 cell survival by WECU was associated with the induction of apoptosis. WECU-induced apoptotic cell death was related to the activation of caspase-8 and -9, representative initiate caspases of extrinsic and intrinsic apoptosis pathways, respectively, and increase in the Bax :Bc1-2 ratio accompanied by cleavage of poly(ADP-ribose) polymerase (PARP). WECU also increased the mitochondrial dysfunction and cytosolic release of cytochrome c. In addition, AMP -activated protein kinase (AMPK) and its downstream target molecule, acetyl-CoA carboxylase, were activated in a concentration dependent manner in WECU-treated cells. In contrast, compound C, an AMPK inhibitor, significantly inhibited WECU-induced apoptosis, while inhibiting increased expression of Bax and decreased expression of Bcl-2 by WECU and inhibition of WECU-induced PARP degradation. Furthermore, WECU provoked the production of reactive oxygen species (ROS); however, the activation of AMKP and apoptosis by WECU were prevented, when the ROS production was blocked by antioxidant N-acetyl cysteine. Therefore, our data indicate that WECU suppresses MCF-7 cell proliferation by activating the intrinsic and extrinsic apoptosis pathways through ROS-dependent AMPK pathway activation. | - |
dc.format.extent | 9 | - |
dc.publisher | PHARMACEUTICAL SOC JAPAN | - |
dc.title | Induction of Apoptosis by Citrus unshiu Peel in Human Breast Cancer MCF-7 Cells: Involvement of ROS-Dependent Activation of AMPK | - |
dc.type | Article | - |
dc.identifier.doi | 10.1248/bpb.b17-00898 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.41, no.5, pp 713 - 721 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000431099200007 | - |
dc.identifier.scopusid | 2-s2.0-85046661616 | - |
dc.citation.endPage | 721 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 713 | - |
dc.citation.title | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.volume | 41 | - |
dc.type.docType | Article | - |
dc.publisher.location | 일본 | - |
dc.subject.keywordAuthor | Citrus unshiu peel | - |
dc.subject.keywordAuthor | breast cancer cell | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | AMP-activated protein kinase | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordPlus | ETHANOL EXTRACT | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | MODEL | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.